Effect of Saccharomyces cerevisiae Live Cells on In Vivo Digestibility and Nitrogen Excretion in Lactating Buffaloes

Authors

  • Felicia Masucci Dipartimento di Agraria, Università di Napoli Federico II, Via Università 100, 80055 Portici (Napoli), Italy
  • Pinar Uzun Dipartimento di Agraria, Università di Napoli Federico II, Via Università 100, 80055 Portici (Napoli), Italy
  • Fernando Grasso Dipartimento di Agraria, Università di Napoli Federico II, Via Università 100, 80055 Portici (Napoli), Italy
  • Giuseppe De Rosa Dipartimento di Agraria, Università di Napoli Federico II, Via Università 100, 80055 Portici (Napoli), Italy
  • Antonio Di Francia Dipartimento di Agraria, Università di Napoli Federico II, Via Università 100, 80055 Portici (Napoli), Italy

DOI:

https://doi.org/10.6000/1927-520X.2014.03.01.4

Keywords:

Buffalo cows, Yeast, Dry matter intake, Digestibility, Fecal nitrogen excretion

Abstract

The effects of dietary inclusion of Saccharomyces cerevisiae culture on intake, in vivo digestibility, and fecal nitrogen excretion were examined in dairy buffalo. Forty lactating buffalo cows were equally divided into Control and Saccharomyces groups, balanced for milk production, parity, and days in milk. Two subsequent 16-d experimental phases were carried out. For both groups during the first experimental period a TMR based on maize silage (maize-TMR) was used, whereas in the second period an alfalfa haylage (alfalfa-TMR) was administered to the animals. In each experimental period, Saccharomyces group was supplemented with 50 g/head/day of yeast (Biocell®, Limena, Padova, Italy), corresponding to 20×109 CFU/head/day Saccharomyces cerevisiae NCYC Sc47 strain. The yeast supplement was top-dressed onto the morning feed. Dry matter intake (DMI) was assessed for 6 consecutive d on group basis, by the difference between feed offered and refused. In the last 3 days of experimental period in vivo digestibility was determined by using acid-insoluble ash (AIA) as an intrinsic digestibility marker. Saccharomyces supplemented buffalo cows presented greater DMI of maize-TMR, whereas no statistical differences between the groups were observed for alfalfa-TMR. Saccharomyces supplementation significantly improved in vivo digestibility of both TMR. Fecal nitrogen excretion was significantly reduced by the use of yeast supplementation. Results suggest that the inclusion of Saccharomyces cerevisiae culture in the diet for lactating buffaloes can be recommended for its effects on cow’s digestive efficiency and fecal nitrogen excretion.

References

ISTAT. Istituto Nazionale di Statistica. 6th General census of agriculture, Rome Italy. 2010.

AIA. Milking recording activity: official statistics 2012. [cited 2014 Jan 15]: Available from: http://www.cd3wd.com/ cd3wd_40/cd3wd/AGRIC/B21WAE/EN/B1723.HTMhttp://bollettino.aia.it/bollettino/Doc/RS_NoteEN.pdf. 2012.

Infascelli R, Faugno S, Pindozzi S, Pelorosso R, Boccia L. The environmental impact of buffalo manure in areas specialized in mozzarella production, southern Italy. Geospatial Health 2010; 5: 131-7.

Desnoyers M, Giger-Reverdin S, Bertin G, Duvaux-Ponter C, Sauvant D. Meta-analysis of the influence of Saccharomyces cerevisiae supplementation on ruminal parameters and milk production of ruminants. J Dairy Sci 2009; 92: 1620-32. http://dx.doi.org/10.3168/jds.2008-1414

Chaucheyras-Durand F, Walker ND, Bach A. Effects of active dry yeasts on the rumen microbial ecosystem: Past, present and future. Anim Feed Sci Technol 2008; 145: 5-26. http://dx.doi.org/10.1016/j.anifeedsci.2007.04.019

Robinson PH, Erasmus LJ. Effects of analyzable diet components on responses of lactating dairy cows to Saccharomyces cerevisiae based yeast products: A systematic review of the literature. Anim Feed Sci Technol 2009; 149: 185-98. http://dx.doi.org/10.1016/j.anifeedsci.2008.10.003

Foley AE, Hristov AN, Melgar A, Ropp JK, Etter RP, Zaman S, et al. Effect of Barley and Its Amylopectin Content on Ruminal Fermentation and Nitrogen Utilization in Lactating Dairy Cows. J Dairy Sci 2006; 89: 4321-35. http://dx.doi.org/10.3168/jds.S0022-0302(06)72479-1

AOAC. Official methods of analysis. (17th ed.). Washington, DC: Association of Official Analytical Chemists 2002.

Van Soest PJ, Robertson JB, Lewis BA. Methods for dietary fiber, neutral detergent fiber, and non starch polysaccharides in relation to animal nutrition. J Dairy Sci 1991; 74: 3583-97. http://dx.doi.org/10.3168/jds.S0022-0302(91)78551-2

Van Keulen J, Young BA. Evaluation of acid-insoluble ash as a natural marker in ruminant digestibility studies. J Anim Sci 1977; 44: 282-7.

Jarrige R. Ed. Ruminant Nutrition: Recommended Allowan-ces and Feed Tables. John Libbey Eurotext 1989; p. 400.

SAS. SAS Institute. SAS/STAT user's guide: version 6. Vol. 2. Sas Inst 1990.

MBC Consortium. Consortium for the Protection of Mozzarella di Bufala Campana PDO - Modello di Regolamento per la gestione igienica ed alimentare dell’allevamento bufalino in relazione alla produzione di mozzarella di bufala campana DOP. Consorzio per la produzione della Mozzarella di Bufala Campana 2002.

Wohlt JE, Finkelstein AD, Chung CH. Yeast Culture to Improve Intake, Nutrient Digestibility, and Performance by Dairy Cattle During Early Lactation. J Dairy Sci 1991; 74: 1395-400. http://dx.doi.org/10.3168/jds.S0022-0302(91)78294-5

Wohlt JE, Corcione TT, Zajac PK. Effect of Yeast on Feed Intake and Performance of Cows Fed Diets Based on Corn Silage During Early Lactation. J Dairy Sci 1998; 81: 1345-52. http://dx.doi.org/10.3168/jds.S0022-0302(98)75697-8

Dann HM, Drackley JK, McCoy GC, Hutjens MF, Garrett JE. Effects of Yeast Culture (Saccharomyces cerevisiae) on Prepartum Intake and Postpartum Intake and Milk Production of Jersey Cows. J Dairy Sci 2000; 83: 123-7. http://dx.doi.org/10.3168/jds.S0022-0302(00)74863-6

Wang Z, Eastridge ML, Qiu X. Effects of Forage Neutral Detergent Fiber and Yeast Culture on Performance of Cows During Early Lactation. J Dairy Sci 2001; 84: 204-12. http://dx.doi.org/10.3168/jds.S0022-0302(01)74470-0

Moallem U, Lehrer H, Livshitz L, Zachut M, Yakoby S. The effects of live yeast supplementation to dairy cows during the hot season on production, feed efficiency, and digestibility. J Dairy Sci 2009; 92: 343-51. http://dx.doi.org/10.3168/jds.2007-0839

Arambel MJ, Kent BA. Effect of Yeast Culture on Nutrient Digestibility and Milk Yield Response in Early- to Midlactation Dairy Cows. J Dairy Sci 1990; 73: 1560-3. http://dx.doi.org/10.3168/jds.S0022-0302(90)78825-X

Soder KJ, Holden LA. Dry Matter Intake and Milk Yield and Composition of Cows Fed Yeast Prepartum and Postpartum. J Dairy Sci 1999; 82: 605-10. http://dx.doi.org/10.3168/jds.S0022-0302(99)75273-2

Al Ibrahim RM, Kelly AK, O’Grady L, Gath VP, McCarney C, Mulligan FJ. The effect of body condition score at calving and supplementation with Saccharomyces cerevisiae on milk production, metabolic status, and rumen fermentation of dairy cows in early lactation. J Dairy Sci 2010; 93: 5318-28. http://dx.doi.org/10.3168/jds.2010-3201

Campanile G, Zicarelli F, Vecchio D, Pacelli C, Neglia G, Balestrieri A, et al. Effects of Saccharomyces cerevisiae on in vivo organic matter digestibility and milk yield in buffalo cows. Livest Sci 2008; 114: 358-61. http://dx.doi.org/10.1016/j.livsci.2007.11.002

Khattab HM, Abo El-Nor SAH, Kholif SM, El-Sayed HM, Abd El-Shaffy OH, Saada M. Effect of different additive sources on milk yield and composition of lactating buffaloes. Livest Sci 2010; 131: 8-14. http://dx.doi.org/10.1016/j.livsci.2010.02.016

Masucci F, De Rosa G, Barone CMA, Parente P, Varricchio ML, Di Francia, et al. Effect of Saccharomyces cerevisiae live cells on milk yield and digestibility of buffalo cows. ADSA-ASAS Joint Annual Meeting. American Dairy Science Association® /American Society of Animal Science; J Dairy Sci 96, E-Supplement 1 2013; 249-50.

Mertens DR. Regulation of forage intake. Forage Qual Eval Util 1994; (foragequalityev): 450-93.

Wallace RJ. Ruminal microbiology, biotechnology, and ruminant nutrition: progress and problems. J Anim Sci 994; 72: 2992-3003.

Di Francia A, Masucci F, De Rosa G, Varricchio ML, Proto V. Effects of Aspergillus oryzae extract and a Saccharomyces cerevisiae fermentation product on intake, body weight gain and digestibility in buffalo calves. Anim Feed Sci Technol 2008; 140: 67-77. http://dx.doi.org/10.1016/j.anifeedsci.2007.02.010

Miranda RLA, Mendoza MGD, Bárcena-Gama JR, González MSS, Ferrara R, Ortega CME, et al. Effect of Saccharomyces cerevisiae or Aspergillus oryzae cultures and NDF level on parameters of ruminal fermentation. Anim Feed Sci Technol 1996; 63: 289-96. http://dx.doi.org/10.1016/S0377-8401(96)01008-5

Wiedmeier RD, Arambel MJ, Walters JL. Effect of Yeast Culture and Aspergillus oryzae Fermentation Extract on Ruminal Characteristics and Nutrient Digestibility. J Dairy Sci 1987; 70: 2063-8. http://dx.doi.org/10.3168/jds.S0022-0302(87)80254-0

Williams PE, Tait CA, Innes GM, Newbold CJ. Effects of the inclusion of yeast culture (Saccharomyces cerevisiae plus growth medium) in the diet of dairy cows on milk yield and forage degradation and fermentation patterns in the rumen of steers. J Anim Sci 1991; 69: 3016-26.

Lehloenya KV, Krehbiel CR, Mertz KJ, Rehberger TG, Spicer LJ. Effects of Propionibacteria and Yeast Culture Fed to Steers on Nutrient Intake and Site and Extent of Digestion1. J Dairy Sci 2008; 91: 653-62. http://dx.doi.org/10.3168/jds.2007-0474

Harrison GA, Hemken RW, Dawson KA, Harmon RJ, Barker KB. Influence of Addition of Yeast Culture Supplement to Diets of Lactating Cows on Ruminal Fermentation and Microbial Populations. J Dairy Sci 1988; 71: 2967-75. http://dx.doi.org/10.3168/jds.S0022-0302(88)79894-X

Putnam DE, Schwab CG, Socha MT, Whitehouse NL, Kierstead NA, Garthwaite BD. Effect of Yeast Culture in the Diets of Early Lactation Dairy Cows on Ruminal Fermentation and Passage of Nitrogen Fractions and Amino Acids to the Small Intestine. J Dairy Sci 1997; 80: 374-84. http://dx.doi.org/10.3168/jds.S0022-0302(97)75947-2

Callaway ES, Martin SA. Effects of a Saccharomyces cerevisiae Culture on Ruminal Bacteria that Utilize Lactate and Digest Cellulose. J Dairy Sci 997; 80: 2035-44.

Chaucheyras F, Fonty G, Bertin G, Gouet P. Effects of live Saccharomyces cerevisiae cells on zoospore germination, growth, and cellulolytic activity of the rumen anaerobic fungus, Neocallimastix frontalis MCH3. Curr Microbiol 1995; 31: 201-5. http://dx.doi.org/10.1007/BF00298373

Piva G, Belladonna S, Fusconi G, Sicbaldi F. Effects of Yeast on Dairy Cow Performance, Ruminal Fermentation, Blood Components, and Milk Manufacturing Properties. J Dairy Sci 1993; 76: 2717-22. http://dx.doi.org/10.3168/jds.S0022-0302(93)77608-0

Enjalbert F, Garrett JE, Moncoulon R, Bayourthe C, Chicoteau P. Effects of yeast culture (Saccharomyces cerevisiae) on ruminal digestion in non-lactating dairy cows. Anim Feed Sci Technol 1999; 76: 195-206. http://dx.doi.org/10.1016/S0377-8401(98)00230-2

Doreau M, Jouany JP. Effect of a Saccharomyces cerevisiae Culture on Nutrient Digestion in Lactating Dairy Cows. J Dairy Sci 1998; 81: 3214-21. http://dx.doi.org/10.3168/jds.S0022-0302(98)75885-0

Guedes CM, Gonçalves D, Rodrigues MAM, Dias-da-Silva A. Effects of a Saccharomyces cerevisiae yeast on ruminal fermentation and fibre degradation of maize silages in cows. Anim Feed Sci Technol 2008; 145: 27-40. http://dx.doi.org/10.1016/j.anifeedsci.2007.06.037

Cornu A, Besle JM, Mosoni P, Grenet E. Lignin-carbohydrate complexes in forages: structure and consequences in the ruminal degradation of cell-wall carbohydrates. Reprod Nutr Dev 1994; 34: 385-98. http://dx.doi.org/10.1051/rnd:19940501

Chesson A. Lignin-polysaccharide complexes of the plant cell wall and their effect on microbial degradation in the rumen. Anim Feed Sci Technol 1988; 21: 219-28. http://dx.doi.org/10.1016/0377-8401(88)90104-6

Campanile G, Neglia G, Vecchio D, Di Palo R, Gasparrini, B, Zicarelli L. Protein nutrition and nitrogen balance in buffalo cows. Animal Science Reviews 2010. CABI; 2011. 1.

Contò G. Riduzione delle emissioni azotate nell’allevamento della bufala da latte. PhD thesis. 2011. [cited 2013 Dec 18] Available from: http: //www.fedoa.unina.it/8826/

Van de Haar MJ, St-Pierre N. Major Advances in Nutrition: Relevance to the Sustainability of the Dairy Industry. J Dairy Sci 2006; 89: 1280-91. http://dx.doi.org/10.3168/jds.S0022-0302(06)72196-8

Downloads

Published

2014-03-21

How to Cite

Masucci, F., Uzun, P., Grasso, F., De Rosa, G., & Di Francia, A. (2014). Effect of Saccharomyces cerevisiae Live Cells on In Vivo Digestibility and Nitrogen Excretion in Lactating Buffaloes. Journal of Buffalo Science, 3(1), 18–24. https://doi.org/10.6000/1927-520X.2014.03.01.4

Issue

Section

Articles