Characterization of Seasonal Variations in Responsiveness of Pituitary Gland to Different Doses of Gonadotropin Releasing Hormone in Buffalo Cows

Authors

  • MEDHAMURTHY RUDRAIAH Department of Molecular Reproduction, Development and Genetics (MR, JUR, KBS, KA and ST), Indian Institute of Science; and National Institute of Animal Nutrition and Physiology (RJP), Bangalore-560012, India
  • U.R. Jyotsna Department of Molecular Reproduction, Development and Genetics (MR, JUR, KBS, KA and ST), Indian Institute of Science; and National Institute of Animal Nutrition and Physiology (RJP), Bangalore-560012, India
  • B.S. Kunal Department of Molecular Reproduction, Development and Genetics (MR, JUR, KBS, KA and ST), Indian Institute of Science; and National Institute of Animal Nutrition and Physiology (RJP), Bangalore-560012, India
  • A. Killivalavan Department of Molecular Reproduction, Development and Genetics (MR, JUR, KBS, KA and ST), Indian Institute of Science; and National Institute of Animal Nutrition and Physiology (RJP), Bangalore-560012, India
  • T. Sudeshna Department of Molecular Reproduction, Development and Genetics (MR, JUR, KBS, KA and ST), Indian Institute of Science; and National Institute of Animal Nutrition and Physiology (RJP), Bangalore-560012, India
  • J.P. Ravindra Department of Molecular Reproduction, Development and Genetics (MR, JUR, KBS, KA and ST), Indian Institute of Science; and National Institute of Animal Nutrition and Physiology (RJP), Bangalore-560012, India

DOI:

https://doi.org/10.6000/1927-520X.2012.01.01.11

Keywords:

GnRH, LH, FSH, Buffalo cows, Holstein-Friesian, Rainy season, Summer season, Hypothalamus, Estrous cyclicity, Artificial insemination.

Abstract

In tropical countries such as India, it has been observed that a number of buffalo cows experience seasonal anestrous during summer months. This might be due to seasonal changes in responsiveness of pituitary gland to gonadotropin releasing hormone (GnRH) and/or decreased hypothalamic GnRH release. Attempts were made to characterize the responsiveness of pituitary gland to a range of doses (0.1, 1, 3, 10 and 33 µg) of GnRH in terms of LH and progesterone (P4) secretions during summer (April-May) and rainy (September-November) months. As a part of these studies, a radioimmunoassay method for estimation of circulating LH in buffalo cows was standardized. During summer months, it was observed that in the presence of low circulating P4 levels the minimum dose of GnRH required for eliciting a significant increase in circulating LH levels was 10 μg/animal that corresponded to a dose of ~28 ng/kg BW. However, during rainy months, administration of the same dose of GnRH failed to elicit a response suggesting that the pituitary gland is not responsive to low doses of exogenous GnRH. On the other hand, buffalo cows receiving a dose of 100 µg of GnRH during rainy months elicited a surge-like increase in circulating LH that peaked at 2 h and the increase in LH concentrations lasted for nearly 6 h post GnRH treatment. The results appear to suggest that during summer months the pituitary gland function is not affected, but there may be lowered hypothalamic GnRH input to the pituitary gland.

References

Clarke IJ. Two decades of measuring GnRH secretion. Reprod Suppl 2002; 59: 1-13.

Rahe CH, Owens RE, Fleeger JL, Newton HJ, Harms PG. Pattern of plasma luteinizing. Endocrinology 1980; 107:

-503. http://dx.doi.org/10.1210/endo-107-2-498

Walters DL, Schallenberger E. Pulsatile secretion of gonadotrophins. J Reprod Fertil 1984; 71: 503-12. http://dx.doi.org/10.1530/jrf.0.0710503

Peters AR, Pimentel MG, Lamming GE. Hormone responses. J Reprod Fertil 1985; 75: 557-65. http://dx.doi.org/10.1530/jrf.0.0750557

Lamming GE, McLeod BJ. Continuous infusion of GnRH. J Reprod Fertil 1988; 82: 237-46. http://dx.doi.org/10.1530/jrf.0.0820237

Vizcarra JA, Wettemann RP, Braden TD, Turzillo AM, Nett TM. Effect of gonadotropin-releasing hormone. Endocrinology 1997; 138: 594-601. http://dx.doi.org/10.1210/en.138.2.594

Peters AR, Lamming GE, Fisher MW. A comparison of plasma LH. J Reprod Fertil 1981; 62: 567-73. http://dx.doi.org/10.1530/jrf.0.0620567

Williams GL, Kotwica J, Slanger WD, Olson DK, Tilton JE, Johnson LJ. Effect of suckling on pituitary responsiveness. J Anim Sci 1982; 54: 594-02.

Richards MW, Wettemann RP, Schoenemann HM. Nutritional anestrus. J Anim Sci 1989; 67: 1520-26.

Riley GM, Peters AR, Lamming GE. Induction of pulsatile LH release. J Reprod Fertil 1981; 63: 559-65. http://dx.doi.org/10.1530/jrf.0.0630559

Spicer LJ, Convey EM, Tucker HA, Echternkamp SE. Effects of intermittent injections of LHRH. J Anim Sci 1986; 62: 1317-23.

Roberge S, Schramm RD, Schally AV, Reeves JJ. Reduced postpartum anestrus. J Anim Sci 1992; 70: 3825-30.

Vizcarra JA, Wettemann RP, Morgan GL. Influence of dose, frequency, and duration of infused gonadotropin-releasing hormone. Domest Anim Endocrinol 1999; 16: 171-81. http://dx.doi.org/10.1016/S0739-7240(99)00007-7

Gwazdauskas FC. Effects of climate. J Dairy Sci 1985; 68: 1568-78. http://dx.doi.org/10.3168/jds.S0022-0302(85)80995-4

Singh J, Nanda AS, Adams GP. The reproductive pattern. Anim Reprod Sci 2000; 60-61: 593-604. http://dx.doi.org/10.1016/S0378-4320(00)00109-3

Wolfenson D, Thatcher WW, Badinga L, et al. Effect of heat stress. Biol Reprod 1995; 52: 1106-13. http://dx.doi.org/10.1095/biolreprod52.5.1106

Wilson SJ, Kirby CJ, Koenigsfeld AT, Keisler DH, Lucy MC. Effects of controlled heat. J Dairy Sci 1998; 81: 2132-8. http://dx.doi.org/10.3168/jds.S0022-0302(98)75789-3

Delasota RL, Burke JM, Risco CA, Moreira F, DeLorenzo MA, Thatcher WW. Evaluation of timed insemination. Theriogenology 1998; 49: 761-70.

Ahuja C, Montiel F, Canseco R, Silva E, Mapes G. Pregnancy rate following GnRH. Anim Reprod Sci 2005; 87: 203-13. http://dx.doi.org/10.1016/j.anireprosci.2004.12.002

Gilad E, Meidan R, Berman A, Graber Y, Wolfenson D. Effect of heat stress. J Reprod Fertil 1993; 99: 315-21. http://dx.doi.org/10.1530/jrf.0.0990315

Aboul-Ela MB, Barkawi AH. Pulsatile secretion of LH in cycling buffalo heifers as affected by season and stage of oestrous cycle. Proceedings of the 11th International Congress on Animal Reproduction and Artificial Insemination; 1988: Dublin; pp. 26-30.

Janakiraman K, Desai MC, Amin DR, Sheth AR, Moodbidri SB, Wadadekar KB. Serum gonadotropin levels. Indian J Anim Sci 1980; 50: 601-6.

Razdan MN, Kaker ML, Galhotra MM. Serum FSH levels.Theriogenology 1982; 17: 175-81. http://dx.doi.org/10.1016/0093-691X(82)90077-2

Komar CM, Berndtson AK, Evans AC, Fortune JE. Decline in circulating estradiol. Biol Reprod 2001; 64: 1797-05. http://dx.doi.org/10.1095/biolreprod64.6.1797

Selvaraj N, Medhamurthy R, Ramachandra SG, Sairam MR, Moudgal NR. Assessment of luteal rescue. J Biosci 1996; 21: 497-500. http://dx.doi.org/10.1007/BF02703214

Sairam MR, Ranganathan MR, Ramasharma K. Binding of an inhibin-like protein. Mol Cell Endocrinol 1981; 22:251-64. http://dx.doi.org/10.1016/0303-7207(81)90095-2

Fuquay JW. Heat stress. J Anim Sci 1981; 52: 164-74.

Francos G, Mayer E. Observations on some environmental. Theriogenology 1983; 19: 625-34. http://dx.doi.org/10.1016/0093-691X(83)90103-6

Clarke IJ, Cummins JT. GnRH pulse frequency. J Reprod Fertil 1985; 73: 425-31. http://dx.doi.org/10.1530/jrf.0.0730425

Edwards S, Roche JF, Niswender GD. Response of suckling beef cows. J Reprod Fertil 1983; 69: 65-72. http://dx.doi.org/10.1530/jrf.0.0690065

Dafopoulos KC, Kotsovassilis CP, Milingos SD, et al. FSH and LH. Clin Endocrinol (Oxf) 2004; 60: 120-4. http://dx.doi.org/10.1111/j.1365-2265.2004.01948.x

Barnes MA, Bierley ST, Halman RD, Henricks DM. Follicle stimulating hormone. Biol Reprod 1980; 22: 459-65. http://dx.doi.org/10.1095/biolreprod22.3.459

McLeod BJ, Haresign W, Peters AR, Lamming GE. Plasma LH and FSH concentrations. J Reprod Fertil 1984; 70: 137-44. http://dx.doi.org/10.1530/jrf.0.0700137

Haughian JM, Ginther OJ, Kot K, Wiltbank MC. Relationships between FSH patterns. Reproduction 2004; 127: 23-33. http://dx.doi.org/10.1530/rep.1.00030

Rao JU, Kunal B. Shah, Jayaram Puttaiah, Medhamurthy Rudraiah. Gene expression. PLoS One 2011; 6(6): e20754. http://dx.doi.org/10.1371/journal.pone.0020754

Karsch FJ, Cummins JT, Thomas GB, Clarke IJ. Steroid feedback. Biol Reprod 1987; 36:1207-18. http://dx.doi.org/10.1095/biolreprod36.5.1207

Brooks J, McNeilly AS. Regulation of gonadotrophin. J Endocrinol 1994; 143: 175-82. http://dx.doi.org/10.1677/joe.0.1430175

Turzillo AM, Clapper JA, Moss GE, Nett TM. Regulation of ovine GnRH. J Reprod Fertil 1998; 113: 251-6. http://dx.doi.org/10.1530/jrf.0.1130251

Turzillo AM, Juengel JL, Nett TM. Pulsatile gonadotropin-releasing hormone. Biol Reprod 1995; 53: 418-23. http://dx.doi.org/10.1095/biolreprod53.2.418

Schoenemann HM, Humphrey WD, Crowder ME, Nett TM, Reeves JJ. Pituitary luteinizing hormone. Biol Reprod 1985; 32: 574-83. http://dx.doi.org/10.1095/biolreprod32.3.574

Richter TA, Robinson JE, Lozano JM, Evans NP. Progesterone can block. J Neuroendocrinol 2005; 17: 161-9. http://dx.doi.org/10.1111/j.1365-2826.2005.01287.x

Garza F, Jr., Thompson DL, Jr., St George RL, French DD. Androgen and estradiol. J Anim Sci 1986; 62: 1654-9.

Sharp DC, Grubaugh WR, Weithenauer J, Davis SD, Wilcox CJ. Effects of steroid administration. Biol Reprod 1991; 44: 983-90. http://dx.doi.org/10.1095/biolreprod44.6.983

Day ML, Imakawa K, Pennel PL, et al. Influence of season. Biol Reprod 1986; 35: 549-53. http://dx.doi.org/10.1095/biolreprod35.3.549

Stumpf TT, Day ML, Wolfe PL, et al. Feedback of 17 beta-estradiol. J Anim Sci 1988; 66: 447-51.

Wolfenson D, Roth Z, Meidan R. Impaired reproduction. Anim Reprod Sci 2000; 60-61: 535-47. http://dx.doi.org/10.1016/S0378-4320(00)00102-0

Schams D, Hofer F, Schallenberger E, Hartl M, Karg H. Pattern of luteinizing hormone. Theriogenology 1974; 1: 137-51.

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Published

2012-02-21

How to Cite

RUDRAIAH, M., Jyotsna, U., Kunal, B., Killivalavan, A., Sudeshna, T., & Ravindra, J. (2012). Characterization of Seasonal Variations in Responsiveness of Pituitary Gland to Different Doses of Gonadotropin Releasing Hormone in Buffalo Cows. Journal of Buffalo Science, 1(1), 61–68. https://doi.org/10.6000/1927-520X.2012.01.01.11

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