Paraconiothyrium sp. P83F4/1: Antioxidant and Antiproliferative Activities an Endophytic Fungus Associated with Rheedia brasiliensis Plant

Brindusa Georgiana Dumitriu; Laura Olariu; Diana Manuela Ene; Lenus Zglimbea; Natalia Rosoiu

doi:10.6000/1927-3037/2012.01.03.04

Authors

Brindusa Georgiana Dumitriu S.C. Biotehnos S.A., Research Department, 3-5 Gorunului Street, Otopeni, Ilfov, 075100 Romania
Laura Olariu S.C. Biotehnos S.A., Research Department, 3-5 Gorunului Street, Otopeni, Ilfov, 075100 Romania
Diana Manuela Ene S.C. Biotehnos S.A., Research Department, 3-5 Gorunului Street, Otopeni, Ilfov, 075100 Romania
Lenus Zglimbea S.C. Biotehnos S.A., Research Department, 3-5 Gorunului Street, Otopeni, Ilfov, 075100 Romania
Natalia Rosoiu Ovidius" University, Faculty of Medicine, Department of Biochemistry, Constanta, Romania; Academy of Romanian Scientists 54 Splaiul Independentei 050094, Bucharest, Romania

DOI:

https://doi.org/10.6000/1927-3037/2012.01.03.04

Keywords:

Crataegus monogyna, UV-radiation, apoptosis, cell cycle, inflammatory cytokines, superoxide anion, catalase, metaloproteinases

Abstract

The endophyte Paraconiothyrium sp. P83F4/1 was isolated from leaves of the Rheedia brasiliensis medicinal plant from a bioprospecting study. To the best of researcher’s knowledge, this is the first report of isolation of Paraconiothyrium in leaves of R. brasiliensis and the fermentation product obtained with this fungus in ethyl acetate was evaluated for their potential bioactive through antioxidant assay by DPPH (radical 1,1-diphenyl-2-picrylhydrazyl) method and antiproliferative assays in human tumoral cells using the sulphorhodamine B technique for evaluating the cellular growth. According to the antioxidant assay, tested at 90 µg/mL (90 ppm), the fungal extract showed scavenging capacity of 58.92 %, being significant (p<0.05) compared to the standard commercial butyl hydroxy toluene: 39.52 %. In the antiproliferative assay, the extract was selective on human keratinocyte cells (HaCat) with GI₅₀ 0.95 µg/mL, whose mean log GI₅₀= - 0.02, according to National Cancer Institute (NCI-USA) criteria, it makes a powerful extract. HaCat Cells are involved directly in diseases such as psoriasis. These results indicate the antioxidant and antiproliferative potentials of the crude extract of fermentation of the endophytic fungus Paraconiothyrium sp. P83F4/1 isolated from the leaves of R. brasiliensis.

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