Analysis of RT-qPCR Data

James R. Perkins; Nor Izayu Abdul Rahman; Matthias Kohl

doi:10.6000/1929-6029.2012.01.02.10

Authors

James R. Perkins Institute of Structural and Molecular Biology, Division of Biosciences, University College London, Gower Street, London, WC1E 6BT, UK
Nor Izayu Abdul Rahman Department of Mechanical and Process Engineering, Furtwangen University, Jakob-Kienzle-Str. 17, D-78054 VS-Schwenningen, Germany
Matthias Kohl Department of Mechanical and Process Engineering, Furtwangen University, Jakob-Kienzle-Str. 17, D-78054 VS-Schwenningen, Germany

DOI:

https://doi.org/10.6000/1929-6029.2012.01.02.10

Keywords:

RT-qPCR, DNA, miRNA, Cq value, amplification efficiency, background correction, normalisation, reference gene, quality control, statistical analysis

Abstract

We give a brief overview of the necessary steps in the analysis of real-time reverse transcription quantitative polymerase chain reaction (RT-qPCR) data. We cover determination of amplification efficiency, background correction, normalization, quality control, and statistical analysis.

Author Biographies

James R. Perkins, Institute of Structural and Molecular Biology, Division of Biosciences, University College London, Gower Street, London, WC1E 6BT, UK

Institute of Structural and Molecular Biology, Division of Biosciences

Nor Izayu Abdul Rahman, Department of Mechanical and Process Engineering, Furtwangen University, Jakob-Kienzle-Str. 17, D-78054 VS-Schwenningen, Germany

Department of Mechanical and Process Engineering

Matthias Kohl, Department of Mechanical and Process Engineering, Furtwangen University, Jakob-Kienzle-Str. 17, D-78054 VS-Schwenningen, Germany

Department of Mechanical and Process Engineering

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