A Comparative Kinetic Study of Free and Immobilized Urease on Commercial and Glutaraldehyde Activated Cotton

M.M. Yousaf, Umer Nasar

Abstract


Urease was immobilized on commercial cotton and commercial cotton activated with glutaraldehyde. The kinetic of urea hydrolysis by free and immobilized urease was studied as a function of pH, temperature and time of hydrolysis. High concentration of ammonia was released at pH 10.0 for free enzyme (0.097 mg/mL) and urease immobilized on cotton (0.092 mg/mL) and pH 4.0 for enzyme, immobilized on activated commercial cotton (0.083 mg/mL). High concentration of ammonia was released at 30 °C for free enzyme (0.006 mg/mL), 25 °C for urease immobilized on cotton (0.043 mg/mL) and urease immobilized on cotton activated with glutaraldehyde (0.015 mg/mL). High concentration of ammonia was released after 10 minutes for free enzyme (0.0016 mg/mL), 60 minutes for inactivated (0.043 mg/mL) and for activated cotton (0.015 mg/ml). The result show that immobilized urease is less effective than the free enzyme and is more active in acid medium than the basic medium.


Keywords


Urease, cotton, immobilization, glutaraldehyde, hydrolysis

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ISSN: 1927-5129