Platelets Mitochondrial Function Depends on Coenzyme Q10 Concentration in Human Young, Not in Elderly Subjects

Authors

  • Anna Gvozdjáková Pharmacobiochemical Laboratory of 3rd Medical Department, Medical Faculty, Comenius University in Bratislava Sasinkova 4, 811 08 Bratislava, Slovakia
  • Zuzana Sumbalová Pharmacobiochemical Laboratory of 3rd Medical Department, Medical Faculty, Comenius University in Bratislava Sasinkova 4, 811 08 Bratislava, Slovakia
  • Jarmila Kucharská Pharmacobiochemical Laboratory of 3rd Medical Department, Medical Faculty, Comenius University in Bratislava Sasinkova 4, 811 08 Bratislava, Slovakia
  • Anežka Chládeková 3rd Medical Department, Medical Faculty, Comenius University in Bratislava, Slovakia
  • Zuzana Rausová Pharmacobiochemical Laboratory of 3rd Medical Department, Medical Faculty, Comenius University in Bratislava Sasinkova 4, 811 08 Bratislava, Slovakia
  • Olga Vancová Pharmacobiochemical Laboratory of 3rd Medical Department, Medical Faculty, Comenius University in Bratislava Sasinkova 4, 811 08 Bratislava, Slovakia
  • Mária Kubalová Pharmacobiochemical Laboratory of 3rd Medical Department, Medical Faculty, Comenius University in Bratislava Sasinkova 4, 811 08 Bratislava, Slovakia
  • Zuzana Kuzmiaková Pharmacobiochemical Laboratory of 3rd Medical Department, Medical Faculty, Comenius University in Bratislava Sasinkova 4, 811 08 Bratislava, Slovakia
  • Michal Nemec Institute of Experimental Endocrinology, Biomedical Research Center Slovak Academy of Sciences, Bratislava, Slovakia
  • Olga Ulicná Pharmacobiochemical Laboratory of 3rd Medical Department, Medical Faculty, Comenius University in Bratislava Sasinkova 4, 811 08 Bratislava, Slovakia
  • Viliam Mojto 3rd Medical Department, Medical Faculty, Comenius University in Bratislava, Slovakia

DOI:

https://doi.org/10.6000/1929-5634.2018.07.03.1

Keywords:

Platelets, mitochondria, High-Resolution Respirometry, coenzyme Q10, age.

Abstract

Ageing is characterized by a progressive decline in the physiological functions of various organs. Mitochondrial alterations occurring in senescence. Antioxidants, including coenzyme Q10 concentration, fall with ageing and contribute to enhanced oxidative stress age-related diseases. The impairment of platelet mitochondrial function occurs in a broad spectrum of diseases.

 The aim of this study was to evaluate mitochondrial function in platelets in elderly and young human controls and correlate it with a concentration of coenzyme Q10. Platelets mitochondrial function was determined by the use of High-Resolution Respirometry method.

We did not find significantly decreased platelet mitochondrial function in elderly subjects. Dependence of platelets mitochondrial respiratory chain function and ATP production at Complex I on a concentration of coenzyme Q10 in platelets and whole blood in young not in elderly human volunteers was documented. This dependence was not found for Complex II in any group. Platelet mitochondrial coenzyme Q10 concentration was insufficient for improving platelet mitochondrial function in elderly human subjects. Recommending supplementation with coenzyme Q10 in elderly and aged humans is waranted.

High-Resolution Respirometry method offers a perspective to diagnose mitochondrial energy metabolism which might be useful for further studies in patients with mitochondrial disorders. Our results could contribute to the explanation of platelets mitochondrial function in elderly and aged human subjects.

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Published

2019-01-18

How to Cite

Gvozdjáková, A., Sumbalová, Z., Kucharská, J., Chládeková, A., Rausová, Z., Vancová, O., Kubalová, M., Kuzmiaková, Z., Nemec, M., Ulicná, O., & Mojto, V. (2019). Platelets Mitochondrial Function Depends on Coenzyme Q10 Concentration in Human Young, Not in Elderly Subjects . Journal of Nutritional Therapeutics, 7(3), 67–76. https://doi.org/10.6000/1929-5634.2018.07.03.1

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