Endophytic Fungi from Aegle marmelos Plant: A Potent and Innovative Platform for Enhanced Cellulolytic Enzyme Production

Authors

  • Pradeep Kumar Badiya Molecular Bioprocessing Laboratory, Department of Chemistry, Sri Sathya Sai Institute of Higher Learning, Prasanthi Nilayam, A.P-515134
  • Sai Praneeth Thota Molecular Bioprocessing Laboratory, Department of Chemistry, Sri Sathya Sai Institute of Higher Learning, Prasanthi Nilayam, A.P-515134
  • Sandeep Yerram Molecular Bioprocessing Laboratory, Department of Chemistry, Sri Sathya Sai Institute of Higher Learning, Prasanthi Nilayam, A.P-515134
  • Praveen V. Vadlani Bioprocessing and Renewable Energy Laboratory, Department of Grain Science and Industry, India; Department of Chemical Engineering, Kansas State University, Manhattan, KS 66506, USA
  • Pallavi Vedantam Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, 10065, USA
  • Sai Sathish Ramamurthy Molecular Bioprocessing Laboratory, Department of Chemistry, Sri Sathya Sai Institute of Higher Learning, Prasanthi Nilayam, A.P-515134
  • Nageswara Rao Golakoti Molecular Bioprocessing Laboratory, Department of Chemistry, Sri Sathya Sai Institute of Higher Learning, Prasanthi Nilayam, A.P-515134
  • Robin Sharma Department of Biosciences, Sri Sathya Sai Institute of Higher Learning, Prasanthi Nilayam Campus, Puttaparthi, Anantapur Dist., Andhra Pradesh
  • B.S. Vijaya Kumar Department of Biosciences, Sri Sathya Sai Institute of Higher Learning, Prasanthi Nilayam Campus, Puttaparthi, Anantapur Dist., Andhra Pradesh

DOI:

https://doi.org/10.6000/1929-6002.2018.07.02

Keywords:

Fermentation, Biomass, Fungi, Extraction, Agricultural Wastes.

Abstract

Fungi have a prominent status in fermentation for the production of different bio-products. Endophytic fungi isolated from medicinal plants are particularly formidable in their adaptability to solid state fermentation as an extension of its natural habitat and are also a potent source of broad-spectrum cellulolytic enzymes. We report for the first time the use of endophytic fungus isolated from Aegle marmelos for enhanced cellulolytic enzymes production from groundnut shell (GNS) as substrate. ImageJ software identified Trichoderma harzianum as an endophytic fungus having maximum radial growth rate. A systematic comparison of the endophytic fungus with Aspergillus oryzae, under solid state fermentation (SSF) and submerged fermentation (SmF) conditions was performed and enhanced cellulase production was observed by the endophytic fungus (4.27 FPU/ml) under SSF environment compared to SmF (2.35 FPU/ml). A comprehensive understanding of the systemic breakdown in the structural integrity of the biomass has been achieved using a synergy of enzyme assay protocols, spectral and thermal based techniques. The use of endophytic fungi in SSF systems in our study lays the basis for the production of other industrially important enzymes. The present study opens the door for the synergistic use of endophytic and epiphytic fungi for the production of cellulolytic enzyme.

References


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2018-08-30

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