Isolation and Characterization of Mannheimia varigena from a Murrah Buffalo

Sarita Yadav; Aman Kumar; Sushila Maan; Ashok Boora; Narender Singh Maan; Rajiv Banger; K.P. Singh; Inderjeet Singh

doi:10.6000/1927-520X.2015.04.01.3

Authors

Sarita Yadav ICAR - Central Institute for Research on Buffaloes (CIRB), Hisar – 125001, India
Aman Kumar Lala Lajpat Rai University of Veterinary & Animal Sciences, Hisar – 125001, India
Sushila Maan Lala Lajpat Rai University of Veterinary & Animal Sciences, Hisar – 125001, India
Ashok Boora ICAR - Central Institute for Research on Buffaloes (CIRB), Hisar – 125001, India
Narender Singh Maan Lala Lajpat Rai University of Veterinary & Animal Sciences, Hisar – 125001, India
Rajiv Banger Trainers Training Institute, Hisar– 125001, India
K.P. Singh ICAR - Central Institute for Research on Buffaloes (CIRB), Hisar – 125001, India
Inderjeet Singh ICAR - Central Institute for Research on Buffaloes (CIRB), Hisar – 125001, India

DOI:

https://doi.org/10.6000/1927-520X.2015.04.01.3

Keywords:

Mannheimia, 16S rRNA sequencing, Murrah buffalo, phenotypic characterization

Abstract

The present study was aimed to isolate and characterize phenotypically as well as genotypically bacterial strain from tracheal froth of a Murrah buffalo on the Government livestock farm (GLF) located at Hisar, Haryana. The isolate showed the cultural, morphological and biochemical properties of Mannheimia haemolytica as per Bergey’s manual of determinative bacteriology and as confirmed by GENIII microplate (biolog). However, the isolate was further confirmed as Mannheimia varigena by 16S rRNA sequencing

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