Gas Chromatographic Mass Spectrometric Analysis of Estrus Specific Volatile Compounds in Buffalo Vaginal Mucus after Initial Sexual Foreplay

kandasamy karthikeyan; Govindaraju Archunan

doi:10.6000/1927-520X.2013.02.01.1

Authors

kandasamy karthikeyan Centre for Pheromone Technology, Department of Animal Science, Bharathidasan University, Tiruchirappalli 620 024, Tamilnadu, India
Govindaraju Archunan Department of Biotechnology and Genetic engineering, Bharathidasan University, Tiruchirappalli 620 024, Tamilnadu, India

DOI:

https://doi.org/10.6000/1927-520X.2013.02.01.1

Keywords:

Estrus, vaginal mucus, Saliva, Buffalo, Oleic acid and GC-MS

Abstract

Vaginal fluid from buffaloes in estrus, non-estrus condition and saliva from bulls that have licked vaginal mucus were collected. The samples were analysed by gas chromatography-mass spectrometry (GC-MS) in order to evaluate the qualitative differences between the volatile profiles of vaginal mucus and saliva to assess their potential value in buffalo bio-communication. Fifteen different organic compounds were detected. The chemical profiles of estrus vaginal fluid were distinguished significantly by the presence of specific substance, 9-octadecenoic acid (oleic acid) that was not present in non-estrus phase. The results are consistent with the view that the oleic acid acts as chemosignal in a number of mammalian species. Based on the result it is concluded that the volatile substance present in buffalo vaginal fluid may act as a chemical stimulant.

Author Biography

Govindaraju Archunan, Department of Biotechnology and Genetic engineering, Bharathidasan University, Tiruchirappalli 620 024, Tamilnadu, India

Department of Animal Science

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