Pregnancy Rates Associated with Oxidative Stress after Estrus Synchronization of Bulgarian Murrah Buffaloes in Breeding and Non-Breeding Season

Radena  Nenova; Yordanka  Ilieva; Pencho  Penchev; Nasko  Vasilev; Ivan  Fasulkov; Galina  Nikolova; Yanka  Karamalakova; Veselina  Gadjeva

doi:10.6000/1927-520X.2023.12.05

Authors

Radena Nenova Department of Obstetrics, Reproduction and Reproductive Disorders, Faculty of Veterinary Medicine, Trakia University, Stara Zagora, Bulgaria
Yordanka Ilieva Agricultural Academy, Agricultural Institute, Shumen, Bulgaria https://orcid.org/0000-0002-2449-9956
Pencho Penchev Agricultural Academy, Agricultural Institute, Shumen, Bulgaria https://orcid.org/0000-0002-3219-0668
Nasko Vasilev Department of Obstetrics, Reproduction and Reproductive Disorders, Faculty of Veterinary Medicine, Trakia University, Stara Zagora, Bulgaria https://orcid.org/0000-0003-2498-7367
Ivan Fasulkov Department of Obstetrics, Reproduction and Reproductive Disorders, Faculty of Veterinary Medicine, Trakia University, Stara Zagora, Bulgaria https://orcid.org/0000-0001-9481-1688
Galina Nikolova Department of Medicinal Chemistry and Biochemistry, Medical Faculty, Trakia University, Stara Zagora, Bulgaria
Yanka Karamalakova Department of Medicinal Chemistry and Biochemistry, Medical Faculty, Trakia University, Stara Zagora, Bulgaria
Veselina Gadjeva Department of Medicinal Chemistry and Biochemistry, Medical Faculty, Trakia University, Stara Zagora, Bulgaria

DOI:

https://doi.org/10.6000/1927-520X.2023.12.05

Keywords:

Oxidative stress, biomarkers, pregnancy rate, Buffaloes, season

Abstract

Background: The current study aims to measure the effect of oxidative stress on the pregnancy rates of Bulgarian Murrah buffaloes during the breeding and non-breeding season.

Methods: The study group consisted of 24 mature buffaloes more than 40 days after parturition. The following parameters were measured: Reactive Oxygen Species (ROS) products, Ascorbate radicals, Malondialdehyde (MDA), Nitric Oxide (NO), Super Oxide Dismutase (SOD), Glutathione peroxidase (GSH-Px), Protein Carbonyl Content (PPC), and total Nitric oxide. The Presynch/Ovsynch protocol was used for estrus synchronization.

Results: A statistically significant increase in ROS products were measured in blood serum during the breeding season compared with the non-breeding season. The highest levels measured were in non-pregnant buffaloes during the breeding season. High levels of oxidative stress were registered due to low SOD activity in buffaloes during the breeding season compared to SOD activity during the non-breeding season. The highest SOD activity was observed in non-pregnant buffaloes during the summer season. The lowest GSH-Px levels were observed in non-pregnant buffaloes during both study periods. During the breeding season, concentrations of total NO and PPC were elevated.

Conclusion: Comparing the obtained results for oxidative stress and antioxidant activity concerning pregnancy rate depending on the season showed that pregnancy in buffaloes during the breeding season was realized at higher values of NO and SOD. Increased oxidative stress was observed, resulting in a statistically significant increase in serum ROS products, as well as decreased SOD activity in buffaloes during the breeding season.

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