Inference about Monophyly of the Family Oedipodidae and the Classification of Subfamilies Based on 16S rDNA Sequences

Authors

  • Dian-Feng Liu Nanjing Normal University
  • Guo-Fang Jiang Nanjing Normal University

DOI:

https://doi.org/10.6000/1927-3037.2014.03.03.4

Keywords:

Oedipodidae, 16S rDNA, monophyly, subfamily.

Abstract

Most of grasshoppers in the family Oedipodidae are the famous agriculture pests in China. However monophyly and the relationships among the subfamilies within this family are unclear up to now. Here the phylogeny of the Oedipodidae was reconstructed based on 16S rDNA sequence fragments by using Mekongiella kingdoni and Atractomorpha sinensis as outgroups under weighted MP, NJ and Bayesian criteria. The 408 bp fragments of mitochondrial 16S rRNA gene were sequenced for 15 species from 4 subfamilies of the family Oedipodidae, and the homologous sequences of other 15 species of grasshoppers were downloaded from the GenBank data library. The numbers of transitions and transversions among pairwise comparisons of the 16S fragments were respectively plotted against percentage sequence differences. Saturation of transitions was discovered, and transversions were not saturated with the increase of percentage sequence difference in the plots. All the individuals of the Oedipodidae excluding Trilophidia annulata were gathered together in the three trees. Our results are very different from the traditionary taxonomy of the Oedipodidae including 4 subfamilies. The Bryodemellinae is not supported as a subfamily, and neither Locustinae nor Oedipodinae are supported as a monophyletic group in this study.

Author Biographies

Dian-Feng Liu, Nanjing Normal University

Biodiversity and Biotechnology

Guo-Fang Jiang, Nanjing Normal University

Biodiversity and Biotechnology

References


[1] Jiang GF, Zheng ZM. Grasshoppers and Locusts from Guangxi. Guangxi Normal University Press, China 1998.
[2] Zheng ZM, Xia KL. Fauna Sinica, Insecta Vol. 10.Orthoptera: Acridoidea (Oedipodidae and Arcypteridae). Beijing: Science Press 1998.
[3] Yin XC. Grasshoppers and locusts from Qingzang Altiplano. Beijing: Science Press 1984.
[4] Uvarov BP. Grasshoppers and locusts, vol. 1. Cambridge University Press, London 1966.
[5] Dirsh VM. Classification of the Acridomorphoidea Insects. Classey, Farringdon, Oxford 1975.
[6] Vickery VR, Kevan DKM. A monograph of the Orthopteroid insects of Canada and adjacent regions. Vol. I-II. Lyman Ent. Museum and Res. Lab. Memoir, No. 13. 1983.
[7] Flook PK, Rowell CHF. The Phylogeny of the Caelifera (Insecta, Orthoptera) as Deduced from mtrRNA Gene Sequences. Mol Phylogenet Evol 1997; 8(1): 89-103. http://dx.doi.org/10.1006/mpev.1997.0412
[8] Liu DF, Jiang GF. Molecular phylogenetic analysis of Acridoidea based on 18S rDNA with a discussion on its taxonomic system. Acta Entomologica Sinica 2005; 48(2): 232-241.
[9] Wang BX, Yang LF. Phylogenetic utilites of mitochondrial DNA sequences in the study of insect systematics. Entomological Knowledge 2002; 39(2): 88-92.
[10] Yin H, Zhang DC, Yin Zh, Liu Y, Yin XC. Molecular phylogeny of some species of the Acridoidea based on 16S rDNA. Acta Genetica Sinica 2003; 30(8): 766-772.
[11] Xia KL. Fauna Sinica, Insecta Vol. 4, Orthoptera. Acridoidea, Pamphagidae, Chrotogonidae, Pyrgomorphidae. Beijing: Science Press 1994.
[12] Sambrook J, Fritsch EF. Molecular Cloning: A Laboratory Manual.Cold Spring Harbor Laboratory 1989.
[13] Simon C, Frati F, Beckenbach A, Crespi B, Liu H, Flook P. Evolution, weighting and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved PCR primers. Ann Entomol Soc Am 1994; 87: 651-701.
[14] Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The Clustal X windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research 1997; 24: 4876-4882. http://dx.doi.org/10.1093/nar/25.24.4876
[15] Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0. Molecular Biology and Evolution 2013; 30: 2725-2729. http://dx.doi.org/10.1093/molbev/mst197
[16] Swofford DL. Phylogenetic Analysis Using Parsimony and other methods, Version 4. Sunderland, MA: Sinauer Associates 1998.
[17] Huelsenbeck, JP, Ronquist F. MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics2001; 17: 754-755. http://dx.doi.org/10.1093/bioinformatics/17.8.754
[18] Flook PK, Klee S, Rowell CHF. Combined molecular phylogenetic analysis of the orthoptera (Arthropoda, Insecta) and implications for their higher systematics. Syst Biol 1999; 48(2): 233-253. http://dx.doi.org/10.1080/106351599260274
[19] Eades DC, Otte D, Cigliano MM, Braun H. Orthoptera Species File Online 2014. Version 5.0/5.0. Available from: http://Orthoptera.Species File.org/ (accessed 10 Oct 2014).
[20] Jia LX, Ma EB. Cytotaxonomic study of some species and genera in Odipodidae. Journal of Shannxi University (Nat. Sci. Ed.). 1998; 21(3): 291296.

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Published

2014-10-15

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