The photo-protective activity of Crataegus monogyna extract proved by complementary mechanisms of skin inflammation, damage of extracellular matrix, cellular survival and proliferation disorders

Authors

  • Brindusa Georgiana Dumitriu Biotehnos
  • Laura Olariu Biotehnos
  • Diana Manuela Ene Biotehnos
  • Lenus Zglimbea Biotehnos
  • Natalia Rosoiu "Ovidius" University, Faculty of Medicine, Department of Biochemistry, Constanta, Romania; Academy of Romanian Scientists

DOI:

https://doi.org/10.6000/1927-3037/2012.01.03.04

Keywords:

UV-A, UV-B, apoptosis, cell cycle, IL6, IL8, IL1α, superoxide anion, hydrogen peroxide, catalase, superoxide dismutase, metaloproteinases

Abstract

The skin photo-ageing and photo-carcinogenesis are based on cellular mechanisms triggered by UV-radiation, starting with oxidative stress and inflammation, activation of matrix-metalloproteinase (MMP) that increase the structural protein degradation, ending with DNA damage and apoptosis induction. In order to claim the photo-protective activity of an ingredient it’s important to explore the main pathways involved in this complex process, highlighting its target action.

Our studies are based on a cellular screening of UV-A and UV-B irradiated keratinocytes (HaCat cell line), focusing on the apoptosis induction, cell cycle sequentiation, metalloproteinase’s expression, pro-inflammatory cytokines release and IL1α as sensitization molecule, as well as the antioxidant capacity of the extract, and acting on catalase and superoxide-dismutase activity. The investigation techniques are flow cytometry (cellular parameters fluorescent staining and beads-based assay for soluble protein detection), zymography and enzymatic activity tests.

The Crataegus monogyna extract, with a rich content of pro-antocians, flavones and polyphenolcarboxylic acids, show a UV-A cellular protection and dose dependent inhibition of inflammatory cytokines and MMP2 and 9 after both UV-A and UV-B irradiation. All these effects are sustained by a cumulative antioxidant capacity at enzymatic and intracellular oxygen radical level.

These findings recommend the phyto-complex for the prevention or therapy of UV-photo damages.

Author Biographies

Brindusa Georgiana Dumitriu, Biotehnos

Research&Development Department

Laura Olariu, Biotehnos

Research&Development Department

Diana Manuela Ene, Biotehnos

Research&Development Department

Lenus Zglimbea, Biotehnos

Research&Development Department

Natalia Rosoiu, "Ovidius" University, Faculty of Medicine, Department of Biochemistry, Constanta, Romania; Academy of Romanian Scientists

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Published

2012-09-19

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Section

Articles