Production of Bioagent for Calcium-Based Biocement
DOI:
https://doi.org/10.6000/1927-3037.2016.05.02.5Keywords:
Bacillus sp., Yaniella sp., Activated sludge, Biocement, BiogroutAbstract
Biocements and biogrouts are developing extensively as new materials alternative to cement and toxic chemical grouts. The most popular type of biocement is a mixture of urease-producing bacteria, urea and calcium salt. Thus, development of biotechnology to produce biomass of urease-active bacteria for large-scale biocementation is an important biotechnological task. Two strains of urease-producing bacteria, Yaniella sp. VS8 and Bacillus sp. VS1 that synthesized inducible and constitutive urease, respectively, were used in the present study. It was shown that low cost biomass of urease-active bacteria can be produced from the hydrolyzed excessive activated sludge of municipal wastewater treatment plant. The biomass of Yaniella sp. VS8 grown in this medium diminished the hydraulic conductivity of sand from 4.8×10-4 m/s to 5∙10-8 m/s after several biotreatments with solution of 1.5 M urea and 0.75M СаCl2.
References
Stabnikov V, Ivanov V, Chu J. Construction Biotechnology: a new area of biotechnological research and applications. World J Microb Biotechnol 2015; 31: 1303-14.http://dx.doi.org/10.1007/s11274-015-1881-7
Al-Thawadi S, Cord-Ruwisch R. Calcium ?arbonate ?rystals formation by ureolytic bacteria isolated from Australian soil and sludge. J Adv Sci Eng Res 2012; 2: 12-26.
Chu J, Stabnikov V, Ivanov V. Microbially induced calcium carbonate precipitation on surface or in the bulk of soil. Geomicrobiol J 2012; 29: 544-9. http://dx.doi.org/10.1080/01490451.2011.592929
De Muynck W, Verbeken K, De Belie N, Verstraete W. Influence of urea and calcium dosage on the effectiveness of bacterially induced carbonate precipitation on limestone. Ecol Eng 99-111.
DeJong JT, Soga KS, Kavazanjian E, Burns S, van Paassen LA, Qabany A, Aydilek A, Bang SS, Burbank M, Caslake L, Chen CY, Cheng X, Chu J et al. Biogeochemical processes and geotechnical applications: progress, opportunities and challenges. Geotechnique 2013; 63: 287-301.http://dx.doi.org/10.1680/geot.SIP13.P.017
Dhami NK Reddy MS, Mukherjee A. Bacillus megateriummediated mineralization of calcium carbonate as biogenic surface treatment of green building materials. World J Microbiol Biotechnol 2013; 29: 2397-406. http://dx.doi.org/10.1007/s11274-013-1408-z
Ivanov V, Chu J. Applications of microorganisms to geotechnical engineering for bioclogging and biocementation of soil in situ.Rev Environ Sci Biotechnol 2008; 7: 139-53.http://dx.doi.org/10.1007/s11157-007-9126-3
Ivanov V, Chu J, Stabnikov V. Basics of construction microbial biotechnology. In: Pacheco-Torgal F, Labrincha JA, Diamanti MV, Chang PY, Lee HK, editors. Biotechnologies and biomimetics for civil engineering. Springer 2015; p. 21-56.
Whiffin VS, van Paassen LA, Harkes MP. Microbial carbonate precipitation as soil improvement technique. Geomicrobiol J 2007; 24: 417-23. http://dx.doi.org/10.1080/01490450701436505
Chou CW, Aydilek AH, Lai M, Seagren EA. Biocalcification of sand through ureolysis. J Geotech Geoenviron 2011; 137: 1179-89.http://dx.doi.org/10.1061/(ASCE)GT.1943-5606.0000532
Li M, Guo H, Cheng X. Application of response surface methodology for carbonate precipitation production induced by a mutant strain of Sporosarcina pasteurii. In: Han J, Alzamora DE, editors. ASCE Geo-Frontiers Adv Geotech Eng 2011; 211: 4079-88.
Mortensen BM, Haber MJ, DeJong JT, Caslake LF, Nelson DC. Effects of environmental factors on microbial induced calcium carbonate precipitation. J Appl Microbiol 2011; 111: 338-49.http://dx.doi.org/10.1111/j.1365-2672.2011.05065.x
Gat D, Tsesarsky M, Shamir D, Ronen Z. Accelerated microbial-induced CaCO3 precipitation in a defined coculture of ureolytic and non-ureolytic bacteria. Biogeosciences 2014; 11: 2561-69.http://dx.doi.org/10.5194/bg-11-2561-2014
Stabnikov V, Chu J, Naeimi M, Ivanov V. Formation of water-impermeable crust on sand surface using biocement. Cement Concrete Res 2011; 41: 1143-49.http://dx.doi.org/10.1016/j.cemconres.2011.06.017
Stabnikov V, Chu J, Ivanov V, Li Y. Halotolerant, alkaliphilic urease-producing bacteria from different climate zones and their application for biocementation of sand. World J Microb Biot 2013; 29: 1453-60. http://dx.doi.org/10.1007/s11274-013-1309-1
Wang JY, Van Tittelboom K, De Belie N, Verstraete W. Potential of applying bacteria to heal cracks in concrete. Proceedings of the Second International conference on sustainable construction materials and technologies; 2010: p. 1807-18.
Hammad IA, Talkhan FN, Zoheir AE. Urease activity and induction of calcium carbonate precipitation by Sporosarcina pasteurii NCIMB 8841. J Appl Sci Res 2013; 9: 1525-33.
Bang SS, Lippert JJ, Yerra U, Mulukutla S, Ramakrishnan V. Microbial calcite, a bio-based smart nanomaterial in concrete remediation. Int J Smart Nano Mater 2010; 1: 28-39.http://dx.doi.org/10.1080/19475411003593451
Gat D, Tsesarsky M, Shamir D, Ronen Z. Accelerated microbial-induced CaCO3 precipitation in a defined coculture of ureolytic and non-ureolytic bacteria. Biogeosciences 2014; 11: 2561-69. http://dx.doi.org/10.5194/bg-11-2561-2014
Al-Thawadi SM. Consolidation of sand particles by aggregates of calcite nanoparticles synthesized by ureolytic bacteria under non-sterile conditions. J Chem Sci Technol2013; 2: 141-6.
Kim HK, Park SJ, Han JI, Lee HK. Microbially mediated calcium carbonate precipitation on normal and lightweight concrete. Constr Build Mater 2013; 38: 1073-82. http://dx.doi.org/10.1016/j.conbuildmat.2012.07.040
Al-Thawadi S. High strength in-situ biocementation of soil by calcite precipitating locally isolated ureolytic bacteria. PhD Thesis, Perth, Western Australia, Murdoch University; 2008.
Bachmeier KL, Williams AE, Warmington JR, Bang SS.Urease activity in microbiologically-induced calcite precipitation.J Biotechnol 2002; 93: 171-81.http://dx.doi.org/10.1016/S0168-1656(01)00393-5
Verma RK, Chaurasia L, Bisht V, Thakur M. Bio-mineralization and bacterial carbonate precipitation in mortar and concrete. Biosci Bioeng 2015; 1: 5-11.
Cheng L, Cord-Ruwisch R. In situ soil cementation with ureolytic bacteria by surface percolation. Ecol Eng 2012; 42: 64-72. http://dx.doi.org/10.1016/j.ecoleng.2012.01.013
Whiffin VS. Microbial CaCO3 precipitation for the production of biocement. PhD thesis, Murdoch University, Perth. 2004.
Achal V, Mukherjee A, Basu PS, Reddy MS. Lactose mother liquor as an alternative nutrient source for microbial concrete production by Sporosarcina pasteurii. J Ind Microbiol Biotechnol 2009; 36: 433-38. http://dx.doi.org/10.1007/s10295-008-0514-7
Nkhalambayausi-Chirwa EM, Lebitso MT. Assessment of nutritional value of single-cell protein from waste-activated sludge as a protein supplement in poultry feed. Water Environ Res 2012; 84: 2106-14.http://dx.doi.org/10.2175/106143012X13415215907130
Belyaev VD, Chepigo SV, Korotchenko NI, Mezentzev AN, Samokhina OV, Krasinskaya, AL, Timkin VN, Shkop YY, Colubey AO. Method for processing activated sludge into useful products. Patent US 4119495A. 1978.
Chu J, Ivanov V, Naeimi M, Stabnikov V, Liu HL. Optimization of calcium-based bioclogging and biocementation of sand.Acta Geotech 2014; 9: 277-85.http://dx.doi.org/10.1007/s11440-013-0278-8
Lane DJ. 16S/23S rRNA sequencing. In: Stackebrandt E, GoodfellowM, editors. Nucleic acid techniques in bacterial systematics. Chichester: John Wiley and Sons. 1991; p. 115-175.
Cao YR, Jiang Y, Jin RX, Han L, He WX, Li YL, Huang XS, Xue QH. Enteractinococcus coprophilus gen. nov., sp. nov., of the family Micrococcaceae, isolated from Panthera tigris amoyensis faeces, and transfer of Yaniella fodinae Dhanjal et al. 2011 to the genus Enteractinococcus as Enteractinococcus fodinae comb. nov. Int J Syst Evol Microbiol 2012; 62: 2710-16.http://dx.doi.org/10.1099/ijs.0.034249-0
Chen YG,Chen J, Chen QH, Tang SK, Zhang YQ, He JW, Li WJ, Liu YQ. Yaniella soli sp. nov., a new actinobacterium isolated from non-saline forest soil in China. Antonie Van Leeuwenhoek 2010; 98: 395-401. http://dx.doi.org/10.1007/s10482-010-9453-1
Dhanjal S, Ruckmani A, Cameotra SS, Pukall R, Klenk HP, Mayilraj S. Yaniella fodinae sp. nov. isolated from a coal mine.Int J Syst Evol Microbiol 2011; 61: 535-39. http://dx.doi.org/10.1099/ijs.0.020636-0
van Paassen L. Biogrout. Ground Improvement by microbially induced carbonate precipitation. PhD Thesis, Delft University of Technology. 2009.
Stocks-Fischer S, Galinat JK, Bang SS. Microbiological precipitation of CaCO3. Soil Biol Biochem 1999; 31: 1563-71.http://dx.doi.org/10.1016/S0038-0717(99)00082-6
Chou CW, Aydilek AH, Lai M, Seagren EA. Biocalcification of sand through ureolysis. J Geotech Geoenviron 2011; 137: 1179-89.http://dx.doi.org/10.1061/(ASCE)GT.1943-5606.0000532
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