Antimicrobial Activity of Vigna unguiculata L. Walp Seed Oil

Mohammad Ashraduzzaman; Mohammad Ashraful Alam; Shahanaz Khatun; Nurul Absar

doi:10.6000/1927-3037.2016.05.03.1

Authors

Mohammad Ashraduzzaman Department of Chemistry, Rajshahi University of Engineering and Technology,
Mohammad Ashraful Alam Department of Chemistry, Rajshahi University of Engineering and Technology,
Shahanaz Khatun Department of Biochemistry and Molecular Biology, University of Rajshahi,
Nurul Absar Department of Biochemistry & Biotechnology, University of Science and Technology

DOI:

https://doi.org/10.6000/1927-3037.2016.05.03.1

Keywords:

Plant oil, seed oil, Vigna unguiculata, antibacteria, antifungi.

Abstract

The antimicrobial activity of three varieties of Vigna unguiculata L. Walp seed oil (LBS-1, LBS-2 and LBS-3) were investigated against five Gram positive bacteria (Bacillus megaterium, Bacillus subtilis, Sarcina lutea, Salmonella typhi and Staphylococcus aureus) and four Gram negative (Escherichia coli, Shigella dysenteriae, Shigella sonnei, Shigella shiga) and four fungi (Penicilium spp., Mucor spp., Candida albicans and Aspergillus fumigatius). The LBS-1 oil at the concentration of 400 µg/ disc showed the highest activity against Sarcina lutea (19±0.1 mm) than that of LBS-2 (14±0.3 mm) and LBS-3 (12±0.3 mm) oil whereas LBS-3 oil showed highest activity against Staphylococcus aureus (16±0.1 mm) than that of LBS-1 (10±0.6 mm) and LBS-2 (13±0.4 mm) oil. All the three oils are active against the three tested fungi namely Penicilium spp., Mucor spp. and Candida albicans but showed no sensitivity against Aspergillus fumigatius.

References

Davis J. Inactivation of antibiotics and the dissemination of resistance gene. Sciences 1994; 264: 375-382.

Kamaran I, Sahin P, Gulluce M, Oguten H, Songul M, Adiguzed A. Antimicroial activity of aqueous and methanol extracts of Juniperus oxycedrus L. J Ethnopharmacol 2003; 2837: 1-5.

Adriana B, Amodovar ANM, Pereira CT, Mariangela TA. Antimicrobial efficacy of Curcuma zedoaria extract as assessed by linear regression compared with commercial mouthrinses. Braz. J Microbiol 2007; 38: 440-445. http://dx.doi.org/10.1590/S1517-83822007000300011

Ho CH, Noryati I, Sulaiman SF, Rosma A. In vitro antibacterial and antioxidant activities of Orthosiphon stamineus Benth. extracts against food-borne bacteria. Food Chemistry 2010; 122: 1168-1172. http://dx.doi.org/10.1016/j.foodchem.2010.03.110

Farrukh U, Shareef H, Mahmud S, Ali SA, Rizwani GH. Antimicrobial activities of Coccinia grandis L. Pak J Bot 2008; 40(3): 1259-1262.

Rahman MM, Seikh MMI, Sharmin SA, Islam MS, Rahman MA, Rahman MM, Alam MF. Antimicrobial activity of leaf juice and extracts of Moringa oleifera Lam. Against some human pathogenic bacteria. CMU J Nat Sci 2009; 8(2): 219- 227.

Alom MA, Habib MR, Nikkon F, Rahman M, Karim MR. Antimicrobial activity of akanda (Calotropis gigantean L.) on some pathogenicbacteria. Bangladesh J Sci Ind Res 2008; 43(3): 397-404.

Dalahaya C, Rainford L, Nicholson A, Mitchel S, Lindo J, Ahmad M. Antibacterial and antifungal analysis of crude extracts from the leaves of Callistemon viminalis. Journal of Medical and Biological Sciences 2009; 3(1): 1-7.

Dewanjee S, Kundu M, Maiti A, Majumdar R, Majumdar A, Mandal SC. In Vitro evaluation of antimicrobial activity of crude extract from plants Diospyros peregrina, Coccinia grandis and Swietenia macrophylla. Trop J Pharm Res 2007; 6: 773-778. http://dx.doi.org/10.4314/tjpr.v6i3.14658

NG NQ, Marechal R. Cowpea taxonomy, origin and germplasm. In Cowpea Research, Production and Utilization. SINGH AND RACHIE (Eds) John Wiley and Sons, New York. 1985; pp. 11-21.

Duke JA. Introduction to food legumes. In: Insect Pests of Tropical Food Legumes, S.R. Singh John Wiley & Sons, (Ed.): Chichester, UK 1990.

Chopra RN, Nayar SI, Chopra IC. Glossary of Indian Medicinal Plants (Including the supplement), New Delhi, Council of Scientific and Industrial Research 1986.

Vanwyk BE, Gericke N. People's Plants: A guide to useful plants of Southern Africa. Briza Publications, Pretoria, South Africa 2000; p. 192.

Ashraduzzaman M, Alam MA, Absar N. A comparative analysis in Physico-Chemical Compositions And Activities of some Enzymes in the three varieties of locally available Barbati (Vigna unguiculata Linn. Walp.) seeds.In. J Sustain Agril Tech 2009; 5(5): 52-56.

Ashraduzzaman M, Alam MA, Khatun S, Banu S, Absar N. Vigna unguiculata Linn. Walp. seed oil exhibiting antidiabetic effects in alloxan induced diabetic rats. Malaysian Journal of Pharmaceutical Sciences 2011; 9(1): 13-23.

Ashraduzzaman M, Alam MA, Khatun S, Luthfunnesa B, Absar N. Extraction and Cherecterization of Cowpea (Vigna unguiculata Linn. Walp.) seed oil. Journal of Science and Technology 2013; 3(2): 431-438.

Hasan NA, Nawahwi MZ, Malek HA. Antimicrobial Activity of Nigella sativa Seed Extract Sains Malaysiana 2013; 42(2): 143-147.

Ibrahim TA, Fagbohun ED. Physicochemical Properties and In vitro Antibacterial activity of Corchorus Olitorius Linn. Seed oil. Life sciences Leaflets 2011; 15: 499-505.

Novak J, Zitterl-Eglseer K, Deans SG, Franz CM. Essential Oils of Different Cultivars of Cannabis sativa L. and their Antimicrobial Activity. Flavour Fragrance Journal 2001; 16(4): 259-262. http://dx.doi.org/10.1002/ffj.993

Kritzinger Q, Lail N, Aveling TAS. Antimicrobial activity of cowpea (Vigna unguiculata) leaf extracts. South African Journal of Botany 2005; 71: 45-48. http://dx.doi.org/10.1016/S0254-6299(15)30147-2

Pena DG, Anguiano RGL, Arredondo JJM. Modification of the method 1 AOAC (CB-method) for the detection of aflatoxins. Bull Environ Contam Toxicol 1992; 49: 485-489. http://dx.doi.org/10.1007/BF00196287

Vander BDA. Vlietnck. Screening methods for antibacterial and antiviral agents from higher plants. IN: Assay for Bioactivity. (K. Hostiettman Academic Press, London) 1991; p. 47-69.

Cowan MM. Plant products as antimicrobial agents. Clin Microbiol Rev 1999; 12(4): 564-82.

Hediat MH, Salama, Najat M. Antimicrobial activity and phytochemical analyses of Polygonum aviculare L. (Polygonaceae), naturally growing in Egypt. Saudi J Biol Sci 2010; 17(1): 57-63. http://dx.doi.org/10.1016/j.sjbs.2009.12.009

Chea A, Jonville MC, Bun SS, Laget M, Elias R, Duménil G, Balansard G. In vitro Antimicrobial Activity of Plants used in Cambodian Traditional Medicine. Am J Chin Med 2007; 35: 867. http://dx.doi.org/10.1142/S0192415X07005338

Lutterodt GD, Ismail A, Bashear RH, Baharudin HM. Antimicrobial effects of Psidium guajava extracts as one mechanism of its antidiarrhoeal action. Malay J Med Sci 1999; 6(2): 17-20.

Hayriye CK, Melissa C. Newman Antimicrobial efficacy of plant phenolic compounds against Salmonella and Escherichia Coli. Food Bioscience 2015; 11: 8-16. http://dx.doi.org/10.1016/j.fbio.2015.03.002

El-Gammal AA, Mansour RM. Antimicrobial activities of some flavonoid compounds. Zentralbl Mikrobiol 1986; 141(7): 561- 5. Antimicrobial Activity of Vigna unguiculata L. Walp Seed Oil International Journal of Biotechnology for Wellness Industries, 2016, Vol. 5, No. 3 75

Aziz NH, Farag SE, Mousa LA, Abo-Zaid MA. Comparative antibacterial and antifungal effects of some phenolic compounds. http://www.ncbi.nlm.nih.gov/pubmed/9670554 1998; 93(374): 43-54.

Esra MMA, Aisha ZIA, Salwa MEK, Umelkheir MAG. Antimicrobial Activity of Cannabis sativa L. Chinese Medicine 2012; 3: 61-64. http://dx.doi.org/10.4236/cm.2012.31010