The Edible Mushroom Pleurotus spp.: II. Medicinal Values


  • Helmi Johari Masri Helmi Johari Masri Institute of Bioproduct Development (IBD), Universiti Teknologi Malaysia (UTM)
  • Parisa Maftoun Parisa Maftoun Institute of Bioproduct Development (IBD), Universiti Teknologi Malaysia (UTM)
  • Roslinda Abd Malek Institute of Bioproduct Development (IBD), Universiti Teknologi Malaysia (UTM)
  • Ali Zineddine Boumehira Centre de Recherche Scientifique et Technique en Analyses Physico Chimiques
  • Avnish Pareek Department of Applied Biotechnology, Ministry of Higher Education, College of Applied Sciences
  • Siti Zulaiha Hanapi Institute of Bioproduct Development (IBD), Universiti Teknologi Malaysia (UTM)
  • Ong Mei Ling Faculty of Chemical Engineering and Energy, Universiti Teknologi Malaysia (UTM)
  • Hesham El Enshasy Institute of Bioproduct Development (IBD), Universiti Teknologi Malaysia (UTM)



Pleurotus spp., medicinal mushrooms, mushrooms bioactive metabolites, immunomodulatory, antimicrobial


Mushrooms health benefit was recognized in the Orient from several thousand years ago as globally consumption and has third largest commercially production. The genus Pleurotus (oyster mushroom) is an organoleptic fast growing fungus which belongs to basidiomycota group. Although 70 species is discovered until now for this genus, but only few of them are available in market such as Pleurotus florida, P. sajor-caju and P. ostreatus. They have been used in human diet throughout the world due to their rich nutrients such as protein, fiber carbohydrate, minerals, vitamins and lipid. It should be noted that low amount calories, Sodium, fat and cholesterol is reported in this genus. Many pharmaceutical companies in the Far East and China are viewing the medicinal mushrooms as a rich source of innovative biomedical molecules. These molecules can be extracted from different part of oyster mushroom such as fruiting body, mycelia and culture broth. Mainly they are biopolymers including polysaccharides, proteins and nucleic acids as they are more variable to carry biological information. The medicinal properties of Pleurotus spp studied by several authors, as a result antitumor, immunomodulating, antiviral, antibacterial, antigenotoxic, antioxidant, anti-inflammatory, hypocholesterolamic, antihypertensive, antiinociceptive, anti-hyperglycaemic, anti-platelet-aggregating, antihepatoma, anti poliferative and antiatherosclerotic. In this chapter, biodivertsity of genus Pleurotus will be discussed regarding nutritional and bioactive compounds. Furthermore the prospective of oyster mushroom for therapeutic application and concept will be highlighted.


[1]Papaspyridi L.-M, Katapodis P, Gonou-Zagou Z, Kapsanaki-Gotsi E, Christakopoulos P. Optimization of biomass production with enhanced glucan and dietary fibres contentby Pleurotus ostreatus ATHUM 4438 under submerged culture. Biochem Eng J 2010; 50(3): 131-8.
[2]Atri N, Sharma SK, Joshi R, Gulati A, Gulati A. Nutritional and neutraceutical composition of five wild culinary-medicinal species of genus Pleurotus (higher Basidiomycetes) from northwest India. Int J Med Meshrooms 2013; 15:49-56.
[3]Eleftherios E, Vassilis MG, Israilidis C. The potential use of mushrooms ?-glucans in the food industry. Int J Biotechnol Well Ind 2014; 3: 15-8.
[4]Mohamed EM, Farghaly FA. Bioactive compounds of fresh and dried Pleurotus ostreatus mushroom. Int J Biotechnol Well Ind 2014; 3: 4-14.
[5]Maftoun P, Johari H, Soltani M, Malik R, Othman NZ, et al. The Edible mushroom Pleurotus spp.: I. Biodiversity and Nutriotional values. Int J Biotechnol Well Ind 2015; 4: 67-83.
[6]Fernandes A, Barros L, Martins A, Herbert P, Ferreira ICFR. Nutritional characterisation of Pleurotus ostreatus (Jacq. Ex Fr.) P. Kumm. Produced using paper scraps as substrate. Food Chem 2015; 169: 396-400.
[7]El Enshasy H, Elsayed EA, Aziz R, Wadaan MA. Mushrooms and Truffles: Historical biofactories for complementary medicine in Africa and in the Middle East. Evidence-Based Compl and Altern Med (eCAM) vol. 2013, Article ID 620451, 10 pages.
[8]Zhang J-J, Li Y, Zhou T, Xu D-P, Zhang P, et al. Bioactivities and health benefits of mushrooms mainly from China. Molecules 2016; 21: 938.
[9]Sharma SK, Gautam N. Chemical, bioactive, and antioxidant potential of twenty wild culinary mushrooms species. Biomed Res Int 2015; 2015: Article ID 346508, 12 pages.
[10]Wu J, Shi S, Wang H, Wang S. Mechanisms underlying the effect of polysaccharides in the treatment of type 2 diabetes: A review. Carbohydr Polymers 2016; 144: 474-94.
[11]Chang ST, Wasser SP. The role of culinary-medicinal mushrooms on human welfare with pyramid model for human health. Int J Med Mushrooms 2012; 12(2): 95-134.
[12]Sarmidi MR, El Enshasy HA. Biotechnology for wellness industry: Concepts and biofactories. Int J Biotechnol Well Ind 2012; 1: 3-28.
[13]Sarangi I, Ghosh D, Bhutia SK, Mallick SK, Maiti TK. Anti-tumor and immunomodulating effects of Pleurotus ostreatusmycelia-derived proteoglycans. Int Immunopharmacol 2006; 6(8): 1287-97.
[14]Jedinak A, Silva D. Pleurotus ostreatus inhibits proliferation of human breast and colon cancer cells through p53-dependent as well as p53 independent pathway. Int J Oncol 2008; 33(6): 1307-13.
[15]Esawy MA, Amer H, Gamal-Eldeen AM, El Enshasy HA, Helmy WA, et al. Scaling up, characterization of levan and its inhibitory role in carcinogenesis initiation stage. Carbohydr Polymers 2013; 95: 578-87.
[16]Maity KK, Patra S, Dey B, Bhunia SK, Mandal S, et al. A heteropolysaccharide from aqueous extract of an edible mushroom, Pleurotus ostreatus cultivar: structural and biological studies. Carbohyd Res 2011; 346(2): 366-72.
[17]Daba A, El-Demellawy M, El Enshasy H. Anticancer activity of plysaccharides produced by Pleurotus ostreatus in submerged culture. In. Genetic and cellular biology of basidiomycetes (Pisabarro AG and Ramirez L Eds.). Universidad Publica de Navarra, Pamplona, Spain 2005; pp. 43-55.
[18]Lavi I, Friesem D, Geresh S, Hadar Y, Shwartz B. An aqueous polysaccharide extract from the edible mushroom Pleurotus ostreatus induces anti-proliferative and pro-apoptotic effects on HT-29 colon cancer cells. Cancer Lett 2006; 244(1): 61-70.
[19]Sun Y, Liu J. Purification, structure and immunobiological activity of a water-soluble polysaccharide from the fruiting body of Pleurotus ostreatus. Bioresour Technol 2009; 100(2): 983-6.
[20]Ren L, Perera C, Hemar Y. Antitumor activity of mushroom polysaccharides: a review. Food Funct 2012; 3: 1118-30.
[21]Daba AS, Ezeronye OU. Anti-cancer effect of polysaccharides isolated from higher basidiomycetes mushrooms. Afr J Biotechnol 2003; 2(12): 672-8.
[22]Tong H, Xia F, Feng K, Sun G, Gao X, et al. Structural characterization and in vitro antitumor activity of a novel polysaccharide isolated from the fruiting bodies of Pleurotus ostreatus. Bioresour Technol 2009; 100(4): 1682-6.
[23]Berne S, Krizaj I, Pohleven F, Turk T, Macek P, et al. Pleurotus and Agrocybe hemolysins, new proteins hypothetically involved in fungal fruiting."" Biochimica et Biophysica Acta (BBA)-General Subjects 2002; 1570(3): 153-9.
[24]Chen J-N, Wang Y-T, Wu J S-B. A glycoprotein extracted from golden oyster mushroom Pleurotus citrinopileatusexhibiting growth inhibitory effect against U937 leukemia cells. J Agric Food Chem 2009; 57(15): 6706-11.
[25]Lv H, Kong Y, Yao Q, Zhang B, Leng F-W, et al. Nebrodeolysin, a novel hemolytic protein from mushroom Pleurotus nebrodensiswith apoptosis-inducing and anti-HIV-1 effects. Phytomed 2009; 16(2-3): 198-205.
[26]Zhang M, Cheung PCK, Chiu LCM, Wong EYL, Ooi VEC. Cell-cycle arrest and apoptosis induction in human breast carcinoma MCF-7 cells by carboxymethylated ?-glucan from the mushroom sclerotia of Pleurotus tuber-regium. Carbohydr Polymers 2006; 66(4): 455-62.
[27]Lavi I, Levinson D, Peri I, Tekoah Y, Hadar Y, et al. Chemical characterization, antiproliferative and antiadhesive properties of polysaccharides extracted from Pleurotus pulmonarius; mycelium and fruiting bodies. Appl Microbiol Biotechnol 2010; 85(6): 1977-90.
[28]Soltani M, Kamyab H, El Enshasy HA. Molecular weight (Mw) and Monosaccharide composition (MC): Two major factors affecting the therapeutic action of polysaccharides extracted from Cordyceps sinensis. J Pure Appl Microbiol 2013; 7: 1601-13.
[29]Zhang M, Cheung PCK, Zhang L, Chiu C-M, Ooi VEC. Carboxymethylated ?-glucans from mushroom sclerotium of Pleurotus tuber-regium as novel water-soluble anti-tumor agent. Carbohydr Polymers 2004; 57(3): 319-25.
[30]Mantovani MS, Bellini MF, Angeli JPF, Oliveira RJ, Silva AF, et al. ?-glucans in promoting health: Prevention against mutation and cancer. Mutation Res 2008; 658: 154-61.
[31]Zhang M, Cheung PCK, Ooi VEC, Zhang L. Evaluation of sulfated fungal ?-glucans from the sclerotium of Pleurotus tuber-regium as a potential water-soluble anti-viral agent. Carbohydr Res 2004; 339(13): 2297-301.
[32]Tao Y, Zhang Y, Zhang L. Chemical modification and antitumor activities of two polysaccharide-protein complexes from Pleurotus tuber-regium. Int J Biol Macromol 2009; 45(2): 109-15.
[33]Wang J-C, Hu S-H, Liang Z-C, Yeh C-J. Optimization for the production of water-soluble polysaccharide from Pleurotus citrinopileatus; in submerged culture and its antitumor effect. Appl Microbiol Biotechnol 2005; 67(6): 759-766.
[34]Zhang J, Wang G, Li H, Zhuang C, Mizuno T, et al. Antitumor polysaccharides from a Chinese mushroom, “Yuhuangmo,’’ the fruiting body of Pleurotus citrinopileatus. Biosci Biotechnol Biochem 1994; 58: 1195-201.
[35]Jeong Y-T, Jeong S-C, Gu Y-A, Islam R, Song C-H. Antitumor and immunomodulating activities of endo-biopolymers obtained from a submerged culture of Pleurotus eryngii. Food Sci Biotechnol 2010; 19(2): 399-404.
[36]Chung K, Choi E, Kim B, Kim Y, Park Y. Studies on the constituents and culture of Korean basidiomycetes. Arch Pharm Res 1982; 5(1): 17-19.
[37]Ngai PHK, Ng TB. A ribonuclease with antimicrobial, antimitogenic and antiproliferative activities from the edible mushroom Pleurotus sajor-caju. Peptides 2004; 25(1): 11-17.
[38]Ngai P, Ng T. A hemolysin from the mushroom Pleurotus eryngii. Appl Microbiol Biotechnol 2006; 72(6): 1185-91.
[39]Li YR, Liu QH, Wang HX, Bg TB. A novel lectin with potent antitumor, mitogenic and HIV-1 reverse transcriptase inhibitory activities from the edible mushroom Pleurotus citrinopileatus. Biochemica et Biophysica Acta (BBA) -General Subjects 2008; 1780(1): 51-7.
[40]Wang H, Gao J, Ng TB. A new lectin with highly potent antihepatoma and antisarcoma activities from the oyster mushroom Pleurotus ostreatus. Biochem Biophys Res Comm 2000; 275(3): 810-6.
[41]Wong JH, Ng TB, Fiang Y, Liu F, Sze W, et al. Purification and characterization of a laccase with inhibitory activity toward HIV-1 reverse transcriptase and tumor cells from an ddible mushroom (Pleurotus cornucopiae). Protein and Peptide Lett 2010; 17(8): 1040-7.
[42]Jedinak A, Dudhgaonkar S, Jiang JH, Sandusky G, Silva D. Pleurotus ostreatus inhibits colitis-related colon carcinogenesis in mice. Int J Mol Med 2010; 26(5): 643-50.
[43]El Enshasy H, Daba A, El Demellawy M, Ibrahim A, El Sayed S, et al. Bioprocess development for large scale production of anticancer exo-polysaccharide by Pleurotus ostreatus in submerged culture. J Appl Sci 2010; 10: 2523-9.
[44]Maftoun P, Malek R, Abbas M, Aziz R, El Enshasy H. Bioprocess for semi-industrial production of immunomodulator polysaccharide Pleuran by Pleurotus ostreatus in submerged culture. J Sci Ind Res 2013; 72: 655-62.
[45]Shamtsyan M, Konusova V, Maksimova Y, Goloshchev A, Panchenko A, et al. Immunomodulating and anti-tumor action of extracts of several mushrooms. J Biotechnol 2004; 113(1-3): 77-83.
[46]Ryu H-S, Kim K-O, Liu Y, Yoon L, Kim H-S. Effects of edible mushrooms (Pleurotus ostreatus (Jacq.) P. Kumm., Pleurotus eryngii, Flammulina velutipes) extracts on immune cell activation in mice. FASEB J 2014; 28: 830.17
[47]El Enshasy H, Daba A, El Demellawy M, Ibrahim A, El Sayed S, El Badry I. Bioprocess development for large scale production of anticancer exo-polysaccharide by Pleurotus ostreatus in submerged culture. J Appl Sci 2010; 10: 2523-9.
[48]Zhang M, Cui SW, Cheung PCK, Wang Q. Antitumor polysaccharides from mushrooms: a review on their isolation process, structural characteristics and antitumor activity. Trends Food Sci Technol 2007; 18(1): 4-19.
[49]Roy SK, Das D, Mondal S, Maiti D, Bhunia B, et al. Structural studies of an immunoenhancing water-soluble glucan isolated from hot water extract of an edible mushroom, Pleurotus florida, cultivar Assam Florida. Carbohydr Res 2009; 344(18): 2596-601.
[50]El Enshasy H, Hatti-Kaul R. Mushroom Immunomodulators: unique molecules with unlimited applications. Trends Biotechnol 2013; 31: 668-77.
[51]Dey B, Bhunia SK, Maity KK, Patra S, Mandal S, et al. Chemical analysis of an immunoenhancing water-soluble polysaccharide of an edible mushroom, Pleurotus florida blue variant. Carbohydr Res 2010; 345(18): 2736-41
[52]Chen JN, Ma CY, Tsai PF, Wang YT, Wu JSB. In vitroAntitumor and Immunomodulatory Effects of the Protein PCP-3A from Mushroom Pleurotus citrinopileatus. J Agric Food Chem 2010. 58(23): 12117-22.
[53]Jesenak M, Majtan J, Rennerova Z, Kyselovic J, Banovcin R, et al. Immunomodulatory effect of pleuran (?-glucan from Pleurotus ostreatus) in children with recurrent respiratory tract infections. Int Immunopharmacol 2013; 15: 395-9.
[54]Vamanue E, Ene M, Vamanu A, Smarandache D, Sabru I, et al. Antioxidant and antibacterial properties of the extracts from Pleurotus ostreatus EVFB1 and EVFB4. Rom Biotechnol Lett 2011; 16(1): 40-6.
[55]Mishra KK, Pal RS, Arunkumar R, Chandrashekara C, Jain SK, et al. Antioxidant properties of different edible mushroom species and increased bioconversion efficiency of Pleurotus eryngii using locally available casting materials. Food Chem 2013; 138: 1557-63.
[56]Jayakumar TM, Sakthivel M, Thomas PA, Geraldine P. Pleurotus ostreatus, an oyster mushroom, decreases the oxidative stress induced by carbon tetrachloride in rat kidneys, heart and brain. Chemico-Biological Interactions 2008; 176(2-3): 108-20.
[57]Jayakumar T, Thomas PA, Geraldine P. In-vitro antioxidant activities of an ethanolic extract of the oyster mushroom, Pleurotus ostreatus. Innovative Food Science & Emerging Technologies 2009; 10(2): 228-34.

[58]Jayakumar T, Thomas PA, Sheu JR, Geraldine P. In-vitro and in-vivo antioxidant effects of the oyster mushroom Pleurotus ostreatus. Food Res Int 2011; 44(4): 851-61.
[59]Bobek P, Ozdín L, Kuniak L. Effect of oyster mushroom and isolated ?-glucan on lipid peroxidation and on the activities of antioxidative enzymes in rats fed the cholesterol diet. The J Nutr Chem 1997; 8(8): 469-71.
[60]Li L, Ng T, Song M, Yuan F, Liu Z, et al. A polysaccharide–peptide complex from abalone mushroom (Pleurotus abalonus) fruiting bodies increases activities and gene expression of antioxidant enzymes and reduces lipid peroxidation in senescence-accelerated mice. Appl Microbiol Biotechnol 2007; 75(4): 863-9.
[61]Jayakumar TE, Ramesh E, Geraldine P. Antioxidant activity of the oyster mushroom, Pleurotus ostreatus, on CCl4-induced liver injury in rats. Food Chem Toxicol 2006; 44(12): 1989-96.
[62]Jose N, Janardhanan KK. Antioxidant and antitumour activity of Pleurotus florida. Curr Sci 2000; 79(7): 941-3.
[63]Xia F, Fan J, Zhu M, Tong H. Antioxidant effects of a water-soluble proteoglycan isolated from the fruiting bodies of Pleurotus ostreatus. J Taiwanese Inst Chem Eng 2011; 42(3): 402-7.
[64]Liu X, Zhou B, Lin R, Jia L, Deng P, et al. Extraction and antioxidant activities of intracellular polysaccharide from Pleurotus sp. mycelium. Int J Biol Macromol 2010; 47(2): 116-9.
[65]Chirinang P, I. Kanok-Orn I. Amino acids and antioxidant properties of the oyster mushrooms, Pleurotus ostreatus and Pleurotus sajor-caju. Science Asia 2009; 35: 326-31.
[66]Lee Y-L, Huang G-W, Liang Z-C, Mau J-L. Antioxidant properties of three extracts from Pleurotus citrinopileatus. LWT – Food Sci Technol 2007; 40(5): 823-33.
[67]Tan YS, Baskaran A, Nallathamby N, Chua KH, Kuppusamy UR, et al. Influence of customized cooking methods on the phenolic contents and antioxidant activities of selected species of oyster mushrooms (Pleurotus spp.). Int J Food Sci Technol 2015; 52: 3058-64.
[68]Bobek P, Ozdín L, Galbavý S. Dose- and time-dependent hypocholesterolemic effect of oyster mushroom (Pleurotus ostreatus) in Rats. Nutrition 1998; 14(3): 282-6.
[69]Choudhury MBK, Mowsumi FR, Mujib TB, Sarker NC, Kabir CMS, et al. Effect of oyster mushroom (Pleurotus ostreatus) on hepatocellular markers alanine aminotransferase and aspartate aminotransferase of adult human during ramadan. Bangladesh J Mushroom 2009; 3(2): 7-11.
[70]Ginterová A, and Janotková O. Utilization of fat and degradation of cholesterol by Pleurotus spp. Folia Microbiol 1981; 26(3): 228-31.
[71]Gunde-Cimerman N, Friedrich J, Cimerman A, Benicki N. Screening fungi for the production of an inhibitor of HMG CoA reductase: Production of mevinolin by the fungi of the genus Pleurotus. FEMS Microbiol Lett 1993; 111(2-3): 203-6.
[72]Gunde-Cimerman N, Plemenitas A, Cimerman A. Pleurotusfungi produce mevinolin, an inhibitor of HMG CoA reductase. FEMS Microbiol Lett 1993; 113(3): 333-7.
[73]Bobek P, Ginter E, Ozdín L. Oyster mushroom (Pleurotusostreatus) accelerates the plasma clearance of low-density and high-density lipoproteins in rats. Nutr Res 1993; 13(8): 885-90.
[74]Bobek P, Ondreicka R, Klvanová J, Ozdín L. Oyster mushroom (Pleurotus ostreatus) decreases serum and liver cholesterol and increases cholesterol 7 ?-hydroxylase activity and fecal excretion of neutral sterols and bile acids in hypercholesterolemic rats. Nutr Res 1994; 14(11): 1683-8.
[75]Hu S-H, Wang J-C, Lien J-L, Liaw E-T, Lee M-Y. Antihyperglycemic effect of polysaccharide from fermented broth of Pleurotus citrinopileatus. Appl Microbiol Biotechnol 2006; 70(1): 107-13.
[76]Kim J-I, Kang M-J, IM J, Seo Y-J, Lee Y-M, et al. Effect of king oyster mushroom (Pleurotus eryngii) on insulin resistance and dyslipidemia in db/db mice. Food Sci Biotechnol 2010; 19(1): 239-42.
[77]Kang TS, Kang MS, Sung JM, Kang AS, Shon HP, et al. Effect of Pleurotus eryngii on the blood glucose and cholesterol in diabetic rats. Korean J Mycrol 2001; 29: 86-90.
[78]Mori K, Kobayashi C, Tomita T, Inatomi S, Ikeda M. Antiatherosclerotic effect of the edible mushrooms Pleurotus eryngii (Eringi), Grifola frondosa (Maitake), and Hypsizygus marmoreus (Bunashimeji) in apolipoprotein E-deficient mice. Nutr Res 2008; 28(5): 335-42.
[79]Alves MJ, Ferreira IC, Dias J, Teixeira V, Martins A, et al. A review on antimicrobial activity of mushroom (Basidiomycetes) extracts and isolated compounds. Planta Med 2012; 78: 1707-18.
[80]Vamanu E. In vitro antimicrobial and antioxidant activity of ethanolic extract of lyophilized mycelium of P. ostreatusPQMZ91109. Molecules 2012; 217: 3653-71.
[81]Oyetayo VO, Ariyo OO. Animicrobial and antioxidant properties of Pleurotus ostreatus (Jacq: Fries) cultivated on different tropical woody substrates. J Waste Conversion Bioprod Biotechnol 2013; 1(2): 28-32.
[82]Wang H, Ng TB. Eryngin, a novel antifungal peptide from fruiting bodies of the edible mushroom Pleurotus eryngii. Peptides 2004; 25(1): 1-5.
[83]Chu KT, Xia L, Ng TB. Pleurostrin: an antifungal peptide from the oyster mushroom. Peptides 2005; 26: 2098-103.
[84]Meng T-X, Ishikawa H, Shimizu K, Ohga S, Kondo R. A glucosylceramide with antimicrobial activity from the edible mushroom Pleurotus citrinopileatus. J Wood Sci 2012; 58(1): 81-6.
[85]Ranghoo-Sanmukhiya MV, Budloo AP, Govinden-Sulange J. Molecular and antibacterial profile of edible oyster mushrooms Pleurotus sajor-caju. Univ of Mauritius Res J 2014; 20: 97-121.
[86]Akyuz M, Onganer P, Erecevit P, Kirbag S. Antimicrobial activity of some edible mushrooms in the eastern and southern region of Turkey. Gazi Univ J Sci 2010; 23:125-30.
[87]Okamoto K, Narayama S, Katsuo A, Shigematus I, Yanase H. Biosynthesis of p-anisaldehyde by the white-rot basidiomycete Pleurotus ostreatus. J Biosci Bioeng 2002; 93(2): 207-10.

[88]Younis AM, Wu F-S, El Shikh HH. Antimicrobial activity of extracts of the oyster culinary medicinal mushroom Pleurotus ostreatus (higher basidiomycetes) and identification of a new antimicrobial compound. Int J Med Mushrooms 2015; 17(6): 579-90.
[89]Martinez MA, Ramirez DO, Simental SS, Perez NR, Mayo MM, et al. Antibacterial activity of spent substrate of mushroom Pleurotus ostreatus enriched with herbs. J Agric Sci 2015; 7(11): 225.
[90]Udu-Ibiam O-E, Ogbu O, Ibiam UA, Nnachi AU. Synergistic antibacterial activity of Pleurotus species (Mushroom) and Psychotria microphylla (Herb) against some clinical isolates. Br J Pharm Res 2015; 7(1): 1-8.
[91]Santoyo S, Ramirez-Anguiano AC, Aldars-Garcia L, Reglero G, Soler-Rivas C. Antiviral activities of Bolletus edulis, Pleurotus ostreatus and Lentinus edodes extracts and polysaccharide fractions against herpes simplex virus type 1. Acta Facultatis Forestallis Zvolen 2012; 51(4): 225-35.
[92]Krupodorova T, Rybalko S, Barshteyn V. Antiviral activity of basidiomycete mycelia against influenza type A (serotype H1N1) and herpes simplex virus type 2 in cell culture. Virol Sin 2014; 29:284-90.
[93]Elsayed EA, El Enshasy HA, Al Wadaan MA, Aziz, R. Mushrooms: A potential natural source of anti-inflammatory compounds for medical applications. Mediators of Inflammation vol. 2014, Article ID 805841, 15 pages
[94]Taofiq O, Martins A, Barreiro MF, Ferreira ICFR. Anti-inflammatory potential of mushroom extracts and isolated metabolites. Trend Food Sci Technol 2016; 50: 193-210.
[95]Smiderle FR, Olsen LM, Carbonero ER, Baggio CH, Freitas CS, et al. Anti-inflammatory and analgesic properties in a rodent model of a (1-3), (1-6)-linked ?-glucan isolated from Pleurotus pulmonarius. Eur J Pharmacol 2008; 597(1-3): 86-91.
[96]Baggio CH, Freitas CS, Martins DF, Mazzardo L, Smiderle FR, et al. Antinociceptive effects of (1-3),(1-6)-Linked beta-glucan isolated fromPleurotus pulmonarius in models of acute and neuropathic pain in mice: evidence for a role for glutamatergic receptors and cytokine pathways. The J of Pain 2010; 11(10): 965-71.
[97]Dandapat S, Sinha MP. Antioxidant and anti-inflammatory activity of Pleurotus tuber-regium (Rumph. Ex Fr.) Singer. Adv Biol Res 2015; 9: 140-5.
[98]Baskaran A, Chua KH, Sabaratnam V, Ram MR, Kuppusamy UR. Pleurotus giganteus (Berk. Karun & Hyde), the giant oyster mushroom inhibits NO production in LPS/H2O2 stimulated RAW 264.7 cells via STAT 3 and COX-2 pathways. BMC Comp Alt Med 2017; 17: 40.
[99]Ghazanfari T, Yaraee R, Farahnejad Z, Rahmati B, Hakimzadeh H. Macrophage activation and nitric oxide alteration in mice treated with Pleurotus florida.Immunopharmacol Immunotoxicol 2010; 31: 47-50.
[100]Choi JH, Kim HG, Jin SW, Han EH, Khanal T, et al. Topical application of Pleurotus eryngii extracts inhibits 2,4-dinitrochlorobenzene-induced atopic dermatitis in NC/Nga mice by the regulation of Th1/Th2 balance. Food Chem Toxicol 2013; 53: 38-45.
[101]Gracher AH, Cipriani TR, Carbonero ER, Gorin PAJ, Iacomini M. Antithrombin and heparin cofactor II-mediated inactivation of alpha-thrombin by a synthetic, sulfated mannogalactan. Thrombosis Res 2010; 126(3): e180-e187
[102]Kim S-W, Kim H-G, Lee B-E, Hwang H-H, Baek D-H, et al. Effects of mushroom, Pleurotus eryngii, extracts on bone metabolism. Clin Nut 2006; 25(1): 166-70.
[103]Jang JH, Lee J, Kim JH, Lee JS. Isolation and identification of RANKL-induced osteoclast differentiation inhibitor from Pleurotus citrinopileatus. Mycosci 2013; 54(4): 265-70.
[104]Rovenský J, Stan?íkova M, Švík K, Bauerová K, Jur?ovi?ová J. The effects of ?-glucan isolated from Pleurotus ostreatuson methotrexate treatment in rats with adjuvant arthritis. Rheumatol Int 2011; 31(4): 507-11.
[105]Khalili M, Ebrahimzadeh MA, Kosaryan M. In vivo iron-chelating activity and phenolic profiles of Angel’s wings mushroom, Pleurotus porrigens (higher basidiomycetes). Int J Med Mushrooms 2015; 17(9): 847-56.
[106]Majtan J, Kumar P, Koller J, Dragunova J, Gbriz J. Induction of metalloproteinase 9 secretion from human keratinocytes by pleuran (?-glucan from Pleurotus ostreatus). Z Naturforsch C 2009; 64(7-8): 597-600.
[107]Lee I-S, Ryoo I-J, Kwon K-Y, Ahn JS, Yoo I-D. Pleurone, a novel human neutrophil elastase inhibitor from the fruiting bodies of the mushroom Pleurotus eryngii var ferulae. J Antibiotics 2011; 64: 587-9.
[108]Taofiq O, Gonzalez-Paramas AM, Martins A, Barreiro MF, Ferreira ICFR. Mushrooms extracts and compounds in cosmetics, cosmeceuticals and nutricosmetics- A review. Ind Crop and products 2016; 90: 38-48.