Fermentation of Carrot Juice by Probiotics: Viability and Preservation of Adhesion

Authors

  • Marja Tamminen Functional Foods Forum, University of Turku, 20014 Turku, Finland
  • Seppo Salminen Functional Foods Forum, University of Turku, 20014 Turku, Finland
  • Arthur C. Ouwehand Functional Foods Forum, University of Turku, 20014 Turku, Finland

DOI:

https://doi.org/10.6000/1927-3037.2013.02.01.2

Keywords:

Lactic acid bacteria, bifidobacteria, vegetables, identification, species specific PCR

Abstract

In the present work, carrot juice was investigated as an alternative carrier for probiotics. Pasteurized juice was fermented anaerobically over night at 37oC with selected probiotics and their viability was assessed after 1, 2, 4, 8 and 12 week storage. Identification was performed by species specific PCR. Mucus adhesion was assessed of carrot juice and laboratory medium grown Lactobacillus strains. At inoculation, the probiotic strains were present at levels of 106-107 CFU/ml and during fermentation the lactobacilli numbers increased to 109-1010 CFU/ml. After four weeks storage, Lactobacillus levels remained almost unchanged and after 12 weeks there were still 107-109 CFU/ml lactobacilli. Bifidobacteria, however, did not grow and started to decline after two weeks and were undetectable after 8 weeks. Adhesion of carrot juice grown lactobacilli was 50-70% less then adhesion of the strains grown in laboratory medium. The results suggest that fermented carrot juice is a promising carrier for probiotic lactobacilli but not bifidobacteria, but may alter the phenotypic properties of lactobacilli.

References

Neish AS. Microbes in Gastrointestinal Health and Desease. Gastroenterology 2009; 136: 65-80. http://dx.doi.org/10.1053/j.gastro.2008.10.080

Högberg A, Lindberg JE. Influence of cereal non-starch polysaccharides and enzyme supplementation on digestion site and gut environment in weaned piglets. Animal Feed Sci Technol 2004; 116: 113-28. http://dx.doi.org/10.1016/j.anifeedsci.2004.03.010

Okada H, Fukushi E, Yamamori A, Kawazoe N, Onodera S, et al. Structural analysis of a novel saccharide isolated from fermented beverage of plant extract. Carbohyd Res 2006; 341: 925-29. http://dx.doi.org/10.1016/j.carres.2006.02.009

de Palencia PF, de la Plaza M, Amárita F, Requena T, Peláez C. Diversity of amino acid converting enzymes in wild lactic acid bacteria. Enzyme Microbial Technol 2006; 38: 88-93.

Dahl S, Tavaria FK, Malcata FX. Relationships between flavour and microbiological profiles in Serra da Estrela cheese throughout ripening. Int Dairy J 2000; 10: 255-62. http://dx.doi.org/10.1016/S0958-6946(00)00042-X

Berdagué JL, Monteil P, Montel MC, Talon R. Effects of starter cultures on the formation of flavour compounds in dry sausage. Meat Sci 1993; 35: 275-87.

Chorianopoulos NG, Boziaris IS, Stamatiou A, Nychas G-JE. Microbial association and acidity development of unheated and pasteurized green-table olives fermented using glucose or sucrose supplements at various levels. Food Microbiol 2005; 22: 117-24. http://dx.doi.org/10.1016/j.fm.2004.04.010

Jung K-O, Park S-Y, Park K-Y. Longer aging time increases the anticancer and antimetastatic properties of doenjang. Nutrition 2006; 22: 539-45. http://dx.doi.org/10.1016/j.nut.2005.11.007

Lin C-H, Wei Y-T, Chou C-C. Enhanced antioxidative activity of soybean koji prepared with various filamentous fungi. Food Microbiol 2006; 23: 628-33. http://dx.doi.org/10.1016/j.fm.2005.12.004

Gardner NJ, Savard T, Obermeier P, Caldwell G, Champagne CP. Selection and characterization of mixed starter cultures for lactic acid fermentation of carrot, cabbage, beet and onion vegetable mixtures. Int J Food Microbiol 2001; 64: 261-75. http://dx.doi.org/10.1016/S0168-1605(00)00461-X

Karovicová J, Drdák M, Greif G, Hybenová E. The choice of strains of Lactobacillus species for the lactic acid fermentation of vegetable juices. Eur Food Res Technol 1999; 210: 53-56.

Rakin M, Vukasinovica M, Siler-Marinkovica S, Maksimovicb M. Contribution of lactic acid fermentation to improved nutritive quality vegetable juices enriched with brewer’s yeast autolysate. Food Chem 2007; 100: 599-602. http://dx.doi.org/10.1016/j.foodchem.2005.09.077

Jägerstad M, Jastrebova J, Svensson U. Folates in fermented vegetables - a pilot study. Lebensmittel-Wissenschaft und - Technologie 2006; 37: 603-11. http://dx.doi.org/10.1016/j.lwt.2003.11.008

Martín R, Olivares M, Marín, ML, Xaus J, Fernández L, et al. Characterization of a reuterin-producing Lactobacillus coryniformis strain isolated from a goat's milk cheese. Int J Food Microbiol 2005; 104: 267-77.

Bergqvist SW, Sandberg A-S, Carlsson NG, Andlid T. Improved iron solubility in carrot juice fermented by homo- and hetero-fermentative lactic acid bacteria. Food Microbiol 2005; 22: 53-61. http://dx.doi.org/10.1016/j.fm.2004.04.006

Gueimonde M, Kalliomäki M, Isolauri E, Salminen S. Probiotic intervention in neonates- will permanent colonization ensue? J Pediatr Gastroenter Nutr 2006; 42: 604-606. http://dx.doi.org/10.1097/01.mpg.0000221897.45910.d3

Lahtinen SJ, Gueimonde M, Reinikainen JP, Ouwehand AC, Salminen SJ. Probiotic bacteria may become dormant during storage. Appl Environm Microbiol 2005; 71: 1662-63. http://dx.doi.org/10.1128/AEM.71.3.1662-1663.2005

Ouwehand AC, Tuomola EM, Tölkkö S, Salminen S. Assessment of adhesion properties of novel probiotic strains to human intestinal mucus. Int J Food Microbiol 2001; 28: 119-26. http://dx.doi.org/10.1016/S0168-1605(00)00440-2

Kun S, Rezessy-Szabó JM, Nguyen QD, Hoschke Á. Changes of microbial population and some components in carrot juice during fermentation with selected Bifidobacterium strains. Process Biochem 2008; 43: 816-21. http://dx.doi.org/10.1016/j.procbio.2008.03.008

Nazzaro F, Fratianni F. Sada A, Orlando P. Synbiotic potential of carrot juice supplemented with Lactobacillus spp. and inulin or fructooligosaccharides. J Sci Food Agric 2008; 88: 2271-76.

Glišić SB, Mišić DR, Stamenić MD, Zizovic IT, Ašanin RM,

et al. Supercritical carbon dioxide extraction of carrot fruit essential oil: Chemical composition and antimicrobial activity. Food Chem 2007; 105: 1346-52.

Yoon KY, Woodams EE, Hang YD. Production of probiotic cabbage juice by lactic acid bacteria. Biores Technol 2006; 97: 1427-30. http://dx.doi.org/10.1016/j.biortech.2005.06.018

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Published

2013-03-31

How to Cite

Tamminen, M., Salminen, S., & Ouwehand, A. C. (2013). Fermentation of Carrot Juice by Probiotics: Viability and Preservation of Adhesion. International Journal of Biotechnology for Wellness Industries, 2(1), 10–15. https://doi.org/10.6000/1927-3037.2013.02.01.2

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