Possible Mutations Expressed in Double Musculature Phenotype

Authors

  • María Angélica Reyes Páez Programa de Medicina Veterinaria, Facultad de Ciencias Agropecuarias, Universidad de la Salle, Bogotá Colombia
  • Natalia Johana Rojas Tovar Programa de Medicina Veterinaria, Facultad de Ciencias Agropecuarias, Universidad de la Salle, Bogotá Colombia
  • Geovanny MendozaSánchez Programa de Medicina Veterinaria, Facultad de Ciencias Agropecuarias, Universidad de la Salle, Bogotá Colombia
  • Marcela Fernández Muñoz Grupo de Investigación REMEAT Reproducción y Mejoramiento en Animales Tropicales Programa de Medicina Veterinaria, Facultad de Ciencias Agropecuarias, Universidad de la Salle, Bogotá Colombia
  • Boris Julián Sepúlveda Molina Biotecnología y Genética S.A (Biotecgen S.A)
  • Andrés Felipe Santander Torres Programa de Medicina Veterinaria, Facultad de Ciencias Agropecuarias, Universidad de la Salle, Bogotá Colombia
  • Jesús Alfredo Berdugo Gutíerrez BIOGEM Facultad de Ciencias Agrarias, Universidad Nacional de Colombia, Sede Medellín Colombia
  • Jhon Didier Ruiz Buitrago Grupo de Investigación INCA-CES. Facultad de Medicina Veterinaria y Zootecnia de la Universidad CES. Medellín Colombia

DOI:

https://doi.org/10.6000/1927-520X.2021.10.01

Keywords:

Myostatin, mutation, double muscle, muscle hyperplasia, Bubalus bubalis.

Abstract

Since the 19th century, the presentation of bovines with disproportionate muscle development have been associated with mutations that inhibit the action of the myostatin gene, it is referred to as double muscle mutation, which is common in some European Bos taurus breeds but it is not reported in buffaloes Bubalus bubalis. This study aims to evaluate if the phenotype observed in 6 young buffaloes with disproportionate muscle development has the same myostatin mutation reported in cattle. DNA was obtained from the blood of the animals of the Murrah breed. First, second and third exon was amplified end point PCR; the fragments were sequenced using capillary electrophoresis. Holstein cattle (Bos taurus) was used As control for normal phenotype. The results obtained from the comparison of the sequence of the myostatin gene show that the observed double-muscled phenotype did not show differences from normal controls. Interspecific variation was demonstrated by comparing exons two and three of the gene, finding 12 variations between the Bos taurus and Bubalus bubalis species in the evaluated fragments. It is necessary to study physiology, and the animals to explain the phenotype observed in buffaloes.

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Published

2021-01-01

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Articles