Estrogenic Endocrine Disruptors and their Possible Deleterious Effects on Marine Organisms: Use of a Novel Monitoring Bioassay
DOI:
https://doi.org/10.6000/1927-3037.2013.02.01.1Keywords:
Endocrine disruption, xenoestrogens, YES, Hermaphrodism, Ruditapes decussates, Cerastoderma glaucumAbstract
Estrogens are both natural and synthetic substances that mimic the effect of the female estrogenic hormone in the body and impart estrogenic activity. Human wastes, birth control pills and chemicals like detergents are a major source of estrogens in the environment. In waste water treatment plants, a major part of these estrogens are not removed after treatment and are released in the marine environment. They are suspected to interfere with the exposed aquatic species’ endocrine systems. In fact, they mimic the effect of the endogenous hormone and therefore can disrupt the endocrine systems of exposed species and the reproductive systems of aquatic fauna. To understand their environmental fate, the estrogenic activity was studied by using the Yeast Estrogenic Screening (YES) bioassay. This bioassay has been validated in the detection of a wide range of estrogenic receptor agonists. A reverse phase HPLC method was used to identify the nature of estrogenic components.We focused on two marine bivalves Ruditapes decussatus and Cerastoderma glaucum recognised bioindicator organisms useful in biomonitoring. Our work is based on in situ and in vivo studies. Different compartments were used: the effluents of a wastewater treatment plant, the sea water, the sediment and the clam Ruditapes decussatus. Some observed histological effects showing hermaphroditic cases and parasites in the cockle Cerastoderma glaucum are also discussed in this paper.
References
EPA. Special report on environmental endocrine disruption: An effects assessment and analysis. (EPA report N° EPA/630/R-96/012). US Environmental Protection Agency, Washington DC 1997.
EEC. Community strategy for endocrine disrupters - a range of substances suspected of interfering with the hormone systems of humans and wildlife. COM 262. Bruxelles 2001; p. 46.
Vos JG, Dybing E, Greim HA, Ladefoed O, Lambré C, Tarazona JV, et al. Health effects of endocrine-disrupting chemicals on wildlife, with special reference to the european situation. Crit Rev Toxicol 2000; 30(1): 71-133. http://dx.doi.org/10.1080/10408440091159176
Guillette LJ Jr, Cross TS, Masson GR, Matter JM, Percival HF, Woodward AR. Developmental abnormalities of the gonad and abnormal sex hormone concentrations in juvenil alligators from contaminated and control lakes in Florida. Environ Health Perspect 1994; 102(8): 680-8. http://dx.doi.org/10.1289/ehp.94102680
Hutchinson JD, Simmonds MP. Organochlorine contamination in pinnipeds. Rev Environ Contam Toxicol 1994; 136: 123-67. http://dx.doi.org/10.1007/978-1-4612-2656-7_4
Wiig O, Derocher AE, Cronin MM, Skarre JU. Female pseudohermaphrodite polar bears at svalbard. J Wildlife Diseases 1998; 34(4): 792-6.
Jobling S, Nolan M, Tyler CR, Brighty G, Sumpter JP. Widespread sexual disruption in wild fish. Environ Sci Technol 1998; 32: 2498-506. http://dx.doi.org/10.1021/es9710870
Minier C, Caltot G, Leboulenger F, Hill EM. An investigation of the incidence of intersex fish in seine-maritime and sussex regions. Analusis 2000; 28: 801-6. http://dx.doi.org/10.1051/analusis:2000280801
Smith BS. Sexuality in the american mud snail (Nassarius obseletus) say. Proc Malacol Soc London 1971; 39: 377-78.
Tillmann M, Schulte-Oehlmann U, Duft M, Markert B, Oehlmann J. Effects of endocrine disruptors on prosobranch snails (Mollusca: Gastropoda) in the laboratory. Part III: Cyproterone acetate and vinclozoline as antiandrogens. Ecotoxicology 2001; 10: 373-88. http://dx.doi.org/10.1023/A:1012279231373
Smith BS. The estuarine mud snail, Nassarius obsoletis: abnormalities in the reproductive system. J Mollus Stud 1980; 46: 247-56.
Gill WB, Schumacher GF, Bibbo M, Straus FH, Schoenberg HW. Association of diethylstilbestrol exposure in utero with cryptorchidism, testicular hypoplasia and semen abnormalities. J Urol 1979; 122(1): 36-9.
Carlsen E, Giwercman A, Keiding N, Skakkebaek NE. Evidence for decreasing quality of semen during past 50 years. BMJ 1992; 305(6854): 609-13. http://dx.doi.org/10.1136/bmj.305.6854.609
Sharpe RM, Skakkebaek NE. Are oestrogens involved in falling sperm counts and disorders of the male reproductive tract? Lancet 1993; 341(8857): 1392-5. http://dx.doi.org/10.1016/0140-6736(93)90953-E
Sharpe RM, Skakkebaek NE. Testicular Dysgenesis Syndrome: Mechanistic insights and potential new downstream Effects. Fertil Steril 2008; 89(2 Suppl): e33-8. http://dx.doi.org/10.1016/j.fertnstert.2007.12.026
Philips DJH. Use of biological indicator organisms to quantitate organochlorine pollutants in aquatic environments. Environ Pollut 1978; 16: 167-29. http://dx.doi.org/10.1016/0013-9327(78)90117-9
Minier C, Abarnou A, Le Guellec AM, Jaouen-Madoulet A, Tutundjian R, Bocquené G, et al. A pollution monitoring pilot study involving chemical analysis and biomarker measurements in the Seine estuary using zebra mussels (Dreissena polymorpha). Environ Toxicol Chem 2006; 25: 112-9. http://dx.doi.org/10.1897/05-161R.1
Oetken M, Bachmann J, Schulte-Oehlmann U, Oehlmann J. Evidence for endocrine disruption in invertebrates. Int Rev Cytol 2004; 236: 1-44. http://dx.doi.org/10.1016/S0074-7696(04)36001-8
Lafont R. The endocrinology of invertebrates. Ecotoxicology 2000; 9: 41-57. http://dx.doi.org/10.1023/A:1008912127592
De Fur PL, Crane M, Ingersoll C, Tattersfield L. Endocrine Disruption in Invertebrates: Endocrinology, Testing and Assessment. Soc Environ Toxicol Chem USA 1999; p. 303.
CGP (Commiseriat Générale de Pèche). Annuaire des statistiques des pêches en Tunisie. Tunis: Ministère de l’agriculture 1996; pp. 1-78.
CRDA. Réseau National de surveillance des zones de production et de commercialisation des mollusques bivalves. 2002. Bilan de la campagne 2001-2002.
Hamza-Chaffai A, Amiard JC, Cosson RP. Relationship between metallothioneins and metals in a natural population of the clam (Ruditapes decussatus) from Sfax coast: a non linear model using Box-Cox transformation. Comp Biochem Physiol 1999; 123: 153-63.
Hamza-Chaffai A, Amiard JC, Pellerin J, Joux L, Berthet B. The potential use of metallothionein in the clam (Ruditapes decussatus) as a biomarker of in situ metal exposure. Comp Biochem Physiol C Toxicol Pharmacol 2000; 127(2): 185-97.
Smaoui-Damak W, Hamza-Chaffai A, Berthet B, Amiard JC. Preliminary study of the clam (Ruditapes decussatus) exposed in situ to metal contamination and originating from the gulf of Gabès, Tunisia. Bull Environ Contam Toxicol 2003; 71: 961-70. http://dx.doi.org/10.1007/s00128-003-8899-5
Gagné F, Blaise C, Pellerin J, Gauthier-Clerc S. Alteration of the biochemical properties of female gonads and vitellins in Mya arenaria at contaminated sites. Mar Environ Res 2002; 53(3): 295-10. http://dx.doi.org/10.1016/S0141-1136(01)00122-2
Gautier-Clerc S, Pellerin J, Blaise C, Gagné F. Delayed gametogenesis of Mya arenaria in the Saguenay Fjord (Canada): a consequence of endocrine disruptors? Comp Biochem Physiol 2002; 131(4): 457-67. doi: 10.1016/S1532-0456(02)00041-8
Siah A, Pellerin J, Benosman A, Gagné JP, Amiard JC. Seasonal gonad progesterone pattern in the soft-shell clam Mya arnaria. Comp Biochem Physiol 2002; 132: 499-11. http://dx.doi.org/10.1016/S1095-6433(02)00095-8
Blaise C, Gagné F, Salazar M, Salazar S, Trottier S, Hansen PD. Experimentally-induced feminization of freshwater mussels after a long-term exposure to a municipal effluent. Fresenius Environ Bull 2003; 12: 865-70.
Osada M, Takamura T, Sato H, Mori K. Vitellogenin synthesis in the ovary of scallop (Patinopecten yessoensis): control by estradiol-17b and the central nervous system. J Exp Zool A Comp Exp Biol 2003; 299(2): 172-9. doi: 10.1002/jez.a.10276
Morcillo Y, Ronis MJJ, Porte C. Effect of tributyltin on the phase I testosterone metabolism and steroid titers of the clam (Ruditapes decussata). Aquatic Toxicol 1998; 42: 1-13. http://dx.doi.org/10.1016/S0166-445X(97)00102-1
Morcillo Y, Porte C. Evidence of endocrine disruption in clams (Ruditapes decussata) transplanted to a tributyltin-polluted environment. Environ Pollut 2000; 107: 47-52. http://dx.doi.org/10.1016/S0269-7491(99)00133-5
Routledge EJ, Sumpter JP. Estrogenic activity of surfactants and some of their degradation products assessed using a recombinant yeast screen. Environ Toxicol Chem 1996; 15: 241-8. http://dx.doi.org/10.1002/etc.5620150303
Damak-Smaoui W, Mathieu M, REBAI T, Hamza-Chaffai A. Histology of the reproductive tissue of the clam Ruditapes decussates. Inverterbrate Reproduction and development 2007; 50: 117-26. doi: 10.1016/j.cbpc.2006.02.009
Ajimi-Macherki M. Validation des biomarqueurs de pollution chez le mollusque Bivalve Cerastoderma glaucum issu du golfe de Gabès : Etude in situ et transplantation in vivo. Thèse de Doctorat, Université de Sfax 2009; p. 200.
Karray S. Etude du cycle reproducteur de la palourde C. glaucum en relation avec les réserves énergétiques (glycogène) en relation sue du golfe de Gabès. Mastère sciences biologiques 2008; p. 64.
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