Current Situation of PC12 Cell Use in Neuronal Injury Study
DOI:
https://doi.org/10.6000/1927-3037.2015.04.02.3Keywords:
Nerve cells, PC12 Cell, Cell differentiation, Neural injury.Abstract
The nervous system diseases are easy to get and hard to cure. The mechanism is bound up with nervous cells injure, so it’s significant to study medicine protect nervous cells injure. We need find an ideal model to study these diseases. PC12 cell is a pheochromocytoma cell line from RattusNorvegicus, because it has some characters of nerve cells and easy to cultivate and passage, these cells have been proved to be a useful cell model to study nervous physiology and pharmacology. There are several of PC12 cells, American type culture collection supply two kinds of PC12 cell named PC12 cell and PC12Adh. There are high differentiation, low differentiation and undifferentiating in domestic. Although they are very similar, there are still some differences, and not every PC12 cell is effective for every experimental model. After compared, we hold that PC12Adh cell line is more suitable for neurite outgrowth studies under ROCK inhibitor than the PC12 cell line, PC12 cells that induced by NGF and high differentiated PC12 cell are similar to cerebral cortical neurons, they are suitable for various physiological and pathological study of nervous system. Undifferentiated PC12 cells due to low levels of dopamine, therefore it is not suitable for study on neural cells.
References
Song HY, Wang ZY, Li NN. Primary cultivation and identification of cortical neuron in neonatal rats. J Appl Clin Pediatr 2012; 27: 129-31. http://dx.doi.org/10.3969/j.issn.1003-515X.2012.02.022
Li CL, Yang BF, Zhang JH, Jiao JD, Huang WS, Wu CF. Primary culture method and identify in neonatal rat hippocampal and cortical neurons. J of Journal of Shenyang Pharm Aceutical University. 2011; 4: 299-304. http://www.cnki.net/KCMS/detail/detail.aspx?QueryID=1&CurRec=3&recid=&filename=SYYD201104015&dbname=CJFD2011&dbcode=CJFQ&pr=&urlid=&yx=&v=MDY4Njg3RGgxVDNxVHJXTTFGckNVUkwrZlllUm9GeUhrVjdyQk5qVFNhckc0SDlETXE0OUVZWVI4ZVgxTHV4WVM=
Qi XM. The protective effects of valproate on cell model of Parkinson's disease. Master Thesis, Suzhou University, Jiangsu, China 2014; 3-18. http://d.wanfangdata.com.cn/ Thesis_D506661.aspx
Greene LA, Tischler AS. Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proc Nat Acad Sci USA 1976; 73: 2424-8. http://dx.doi.org/10.1073/pnas.73.7.2424
ATCC. CRL-1721product details [EB/OL]. [updated 2015 June 27]. http://www.atcc.org/Products/All/CRL-1721. aspx?geo_country=cn#generalinformation
ATCC. CRL-1721.1productdetails [EB/OL]. [updated 2015 June 27]; http://www.atcc.org/Products/All/CRL-1721.1.aspx#generalinformation
Que L, Duan WG, Zhang LY, Jiang ZZ. Differences of neurite outgrowth induced by rho kinase inhibitors between in PC12 cell line and PC12Adh cell line. J of Yunnan Univ (Natural Sciences) 2011; 33: 370-2. http://www.cnki.net/KCMS/ detail/detail.aspx?QueryID=0&CurRec=1&recid=&filename=YNDZ201103024&dbname=CJFD2011&dbcode=CJFQ&pr=&urlid=&yx=&v=MDM1NDhIOURNckk5SFlJUjhlWDFMdXhZUzdEaDFUM3FUcldNMUZyQ1VSTCtmWWVSb0Z5L2tXNzNKUENQUGRMRzQ=
Liu XY. Ghrelin inhibits high glucose-induced PC12 cells apoptosis and its molecular mechanisms. Master Thesis, Chongqing Medical Univ, Chongqing, China 2014; 38-42. http://d.wanfangdata.com.cn/Thesis_Y2689258.aspx
Terzi YK, Kocaefe YC, Ayter S, Kocaefe, Ayter S. Coronin 1A inhibits neurite outgrowth in PC12 cells. J Neurosci Lett 2014; 582: 38-42. http://dx.doi.org/10.1016/j.neulet.2014.08.044
Tamplenizza M, Lenardi C, Maffioli E, et al. Nitric oxide synthase mediates PC12 differentiation induced by the surface topography of nanostructured TiO2. J Nanobiotechnol 2013; 11: 35. http://dx.doi.org/10.1186/1477-3155-11-35
Phan CW, Wong WL, David P, Naidu M, Sabaratnam V. Pleurotusgiganteus (Berk.) Karunarathna & K. D. Hyde: Nutritional value and in vitro neurite outgrowth activity in rat pheochromocytoma cells. J BMC Complement Altern Med 2012; 12: 102. http://dx.doi.org/10.1186/1472-6882-12-102
Duan W, Que L, Lv X, Li Q, Yin H, Zhang L. Tolerance of neurite outgrowth to Rho kinase inhibitors decreased by cyclooxygenase-2 inhibitor. J NeurRegen Res 2012; 7: 2705-12. http://dx.doi.org/10.3969/j.issn.1673-5374.2012.34.008
Yin H, Hou X, Tao T, Lv X, Zhang L, Duan W. Neurite outgrowth resistance to rho kinase inhibitors in PC12Adh cell. J Cell BiolInternat 2015; 1-14. http://dx.doi.org/10.1002/cbin.10423
Krug AW, Langbein H, Ziegler CG, Bornstein SR, Eisenhofer G, Ehrhart-Bornstein M. Dehydroepiandrosterone-sulphate (DHEA-S) promotes neuroendocrine differentiation of chromaffin pheochromocytoma PC12 cells. J Molec Cell Endocrinol 2009; 300: 126-31. http://dx.doi.org/10.1016/j.mce.2008.10.026
Lin YM, Hall RA, Kuhar MJ. CART peptide stimulation of G protein-mediated signaling in differentiated PC12 Cells: Identification of PACAP 6-38 as a CART receptor antagonist. J Neuropeptides 2011; 45: 351-8. http://dx.doi.org/10.1016/j.npep.2011.07.006
Wang GQ. Proteomics of the differentiation of PC12 cells and Cos-7 stable cell line express TrkA under the stimulation of nerve growth factor and protective effect of melatonin on dopaminegic neurons. Doctoral Dissertation, the Second Military Medical Univ, Shanghai, China 2003; 10-37. http://dx.doi.org/10.7666/d.y557298
Zhao WJ, Wei L, He JH, Yang YM. Evaluation of the controlled release of NGF from genipin-crosslinked chitosan membranes immobilized with NGF. Med J Comm 2010; 24: 220-2. http://dx.doi.org/10.3969/j.issn.1006-2440.2010.03.002
Meng L, Jiang A, Chen R, et al. Inhibitory effects of multiwall carbonnanotubes with high iron impurity on viability and neuronal differentiation in cultured PC12 cells. J Toxicol 2012; 313: 49-58. http://dx.doi.org/10.1016/j.tox.2012.11.011
Wang XL, Chen D, Liu JM. Differentiation of PC12 cells into neurons induced by NGF. J of Jilin University (Medicine Edition), Jilin, China 2007; 33: 827-30. http://dx.doi.org/10.3969/j.issn.1671-587X.2007.05.013
Zhang W, Zhang BF, Pen FF, Wu CD. PC12 cells neuron differentiation induced by NGF. J of Jianghan University (Natural Sciences) 2008; 36: 68-72. http://dx.doi.org/10.3969/j.issn.1673-0143.2008.03.021
Jiang H. Mechanism Study of Colistin Sulfate Induced Apposite in PC12 Cells. Northeast Agricultural University. Doctoral Dissertation, HeiLongjiang, China 2013; 111. http://www.cnki.net/kcms/detail/detail.aspx?filename=1013207789.nh&dbcode=CDFD&dbname=CDFD2013&v
Shi LJ, Wang K, Liu LA, Wang CA. The properties and sensitivity to acetylcholine of PC12 cells differentiated with NGF. Chin J Appl Physiol 2003; 19: 74-7. http://dx.doi.org/10.3969/j.issn.1000-6834.2003.01.018
Yang X, Liu X, Zhang X, Lu H, Zhang J, Zhang Y. Investigation of morphological and functional changes during neuronal differentiation of PC12 cells by combined Hopping Probe Ion Conductance Microscopy and patch-clamp technique. J Ultramicroscopy 2011; 111: 1417-22. http://dx.doi.org/10.1016/j.ultramic.2011.05.008
El Omri A, Han J, Yamada P, Kawada K, Ben Abdrabbah M, Isoda H. Rosmarinus officinalis polyphenols activate cholinergic activities in PC12 cells through phosphorylation of ERK1/2. J Ethnopharmacol 2010; 131: 451-8. http://dx.doi.org/10.1016/j.jep.2010.07.006
van Grunsven LA, Thomas A, Urdiales JL, et al. Nerve growth factor-induced accumulation of PC12 cells expressing cyclin D1: evidence for a G phase block. J Oncogene1996; 12: 855-62. http://www.ncbi.nlm.nih.gov/pubmed/?term= 8632908
Rudkin BB, Lazarovici P, Levi BZ, Abe Y, Fujita K, Guroff G. Cell cycle-specific action of nerve growth factor in PC12 cells: differentiation without proliferation. EMBO J 1989; 8: 3319-25. http://www.ncbi.nlm.nih.gov/pubmed/?term= 2555160
Lv SH, Yang Y, Liu SJ. An investigation on the division of neuronal PC12 cells induced by nerve growth factor. J of Sheng Li Xue Bao, 2005; 57: 552-6. http://www.ncbi.nlm. nih.gov/pubmed/16220192
Zhou H, Wang XJ, Weng ZJ, Ma J, Li JQ. Inhibition of (1R, 2R)-SIPI5358 and SIPI5358 on delayed rectifier potassium current in undifferentiated PC12 Cells. Chin J Pharmaceut 2012; 43: 443-7. http://dx.doi.org/10.3969/j.issn.1001-8255.2012.06.013
Li LH. Directly Induction of bone mesenchymal stem cells to neuron-like cells and preparation of the cell models of Parkinson's disease. Master Thesis, Yangzhou Univ, Jiangsu, China 2014; 35. http://d.wanfangdata.com.cn/ Thesis_Y2632257.aspx
Wen JL. The study of the protective effect of phenols of Gastrodiaelata Blume on oxidative damage of PC12 cells induced by H2O2. Master Thesis, Yunnan Univ of Traditional Chinese Medicine 2013; 21. http://d.wanfangdata.com.cn/ Thesis_Y2400584.aspx
Cao R. Role of α-synuclein-tau interaction in manganese-induced neurotoxicity and protective effect of genistein. Doctoral Dissertation, the Fourth Military Medical University, Xi’an, China 2013; 42. http://dx.doi.org/10.7666/d.D357289
Chen X. ALS-associated mutations of protein SOD1-G93A induced injury in PC12 cell. Master Thesis, Hebei Medical University, Hebei, China 2014; 10. http://d.wanfangdata. com.cn/Thesis_Y2583348.aspx
Song YP, Zhang P, Wang XW. The significance of the L-dopa induced PC12 cell apoptosis experiment in the mechanism of L-dopa treatment for Parkinson’s disease effects fade (wearing-off). J Modern Prevent Med 2013; 40: 737-9. http://www.cnki.net/KCMS/detail/detail.aspx? QueryID=0&CurRec=1&recid=&filename=XDYF201304064&dbname=CJFD2013&dbcode=CJFQ&pr=&urlid=&yx=&v=MjY5NjIxTHV4WVM3RGgxVDNxVHJXTTFGckNVUkwrZlllUm9GeXpoVkw3TlBTblNhTEc0SDlMTXE0OURZSVI4ZVg
Hu D, Wang N, Zhang ZT, Zhang ZH. Protective effect of curcumin against rotenone-induced toxicity in NGF-differentiaed PC12 cells. J Stroke and Nervous Dis 2011; 18: 327-8. http://dx.doi.org/10.3969/j.issn.1007-0478.2011.06.002
Zhou JK. The molecular pathways of morphine regulating thioredoxin expression. Master Thesis, Kunming University of Science and Technology, Yunnan, China, 2014:18-57. http://www.cnki.net/KCMS/detail/detail.aspx?QueryID=0&CurRec=1&recid=&filename=1014353173.nh&dbname=CMFD201501&dbcode=CMFD&pr=&urlid=&yx=&v=MTk5MTJGeS9rVTczSVZGMjZHckM5SGRETHJKRWJQSVI4ZVgxTHV4WVM3RGgxVDNxVHJXTTFGckNVUkwrZlllUm8
Kong SZ. Anti-aging effects of hydroxysaffloryellow A. Doctoral Dissertation, Guangzhou University of Chinese Medicine, Guangdong, China 2014; 8-26. http://d.wanfangdata.com.cn/Thesis_Y2622798.aspx
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