Localization Analysis of Natural Toxin of Solanum tuberosum L. via Mass Spectrometric Imaging


  • Riho Hashizaki Department of Bioscience, Fukui Prefectural University
  • Hanaka Komori Department of Bioscience, Fukui Prefectural University
  • Kohei Kazuma Institute of Natural Medicine, University of Toyama
  • Katsuhiro Konno Institute of Natural Medicine, University of Toyama
  • Kyuichi Kawabata Department of Bioscience, Fukui Prefectural University
  • Daisaku Kaneko Kyushu Institute of Technology
  • Hajime Katano Department of Bioscience, Fukui Prefectural University
  • Shu Taira Department of Bioscience, Fukui Prefectural University




Food safety, glycoalkaloid, mass spectrometry imaging, a-solanine, a-chaconine.


The use of mass spectrometry imaging (MSI) revealed the localization of alpha-solanine and alpha-chaconine as natural toxins for Potato (Solanum tuberosum L.). The content of Potato glycoalkaloids, alpha-solanine and alpha-chaconine, were quantitatively determined by high performance liquid chromatography (HPLC). Matrix assisted laser desorption/ionization-based tandem mass spectrometry (MS) could determine alpha-solanine and alpha-chaconine from raw potato extraction and section. After budbreak, alpha-solanine and alpha-chaconine were produced and localized at periderm and germ compared with that before budbreak. At germ region, these glycoalkaloids did not exist whole germ region but eccentrically localize at germ surface and central region. The amount of alpha-chaconine was twofold higher than alpha-solanine at periderm. At germ region, there was no difference between these toxins.


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