Mass spectrometry imaging of the capsaicin localization in the capsicum fruits

Shu Taira; Shuichi Shimma; Issey Osaka; Daisaku Kaneko; Yuko Ichiyanagi; Ryuzo Ikeda; Yasuko Konishi-Kawamura; Shu Zhu; Koichi Tsuneyama; Katsuko Komatsu

doi:10.6000/1927-3037.2012.01.01.04

Mass spectrometry imaging of the capsaicin localization in the capsicum fruits

Authors

Shu Taira JAPAN Advanced Institute of Science and Technology
Shuichi Shimma Osaka University
Issey Osaka Yokohama City University
Daisaku Kaneko JAPAN Advanced Institute of Science and Technology
Yuko Ichiyanagi Yokohama National University & Japan Science and Technology Agency
Ryuzo Ikeda Japan Advanced Institute of Science and Technology
Yasuko Konishi-Kawamura Ishikawa Prefectural University
Shu Zhu Institute of Natural Medicine University of Toyama
Koichi Tsuneyama Faculty of Medicine University of Toyama
Katsuko Komatsu Institute of Natural Medicine University of Toyama

DOI:

https://doi.org/10.6000/1927-3037.2012.01.01.04

Keywords:

capsaicin, mass spectrometry, imaging, capsicum, placenta

Abstract

We succeeded in performing mass spectrometry imaging (MSI) of the localization of capsaicin in cross-sections of the capsicum fruits at a resolution of 250 Âµm using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. Post source decay of protonated capsaicin ion revealed structural information of the corresponding acid amide of vanillylamide and C₉ chain fatty acid. MALDI-TOF-MSI confirmed that localization of capsaicin in the placenta is higher than that in the pericarp. In addition, it revealed no localization of capsaicin in seed and the higher localization of capsaicin at placenta surface compared with that in the internal region. A quantitative difference was detected between localizations of capsaicin at placenta, pericarp and seed in the capsicum fruits. This imaging approach is a promising technique for rapid quality evaluation general food as well as health food and identification of medicinal capsaicin in plant tissues.

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Published

2012-04-04

How to Cite

Taira, S., Shimma, S., Osaka, I., Kaneko, D., Ichiyanagi, Y., Ikeda, R., Konishi-Kawamura, Y., Zhu, S., Tsuneyama, K., & Komatsu, K. (2012). Mass spectrometry imaging of the capsaicin localization in the capsicum fruits. International Journal of Biotechnology for Wellness Industries, 1(1), 61–66. https://doi.org/10.6000/1927-3037.2012.01.01.04

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Issue

Vol. 1 No. 1 (2012)

Section

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