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journal-basic-applied-scien

Detection of Grapevine Fanleaf Virus Using Serological and Biological Assays
Pages 343-347
Gülcan Tarla and Mehmet Asil Yilmaz

DOI: http://dx.doi.org/10.6000/1927-5129.2015.11.49

Published: 25 May 2015

Open Access

Abstract: The production of grapevine is important agricultural sector in Turkey. But, the production is lower than the other Mediterranean countries. One of the main reasons of low yield is many viruses’ diseases. The nepoviruse diseases are responsible for significant lossesin vineyards. Grapevine fanleaf virus (GFLV) is the most important deadly virus among them. It is transmitted by graft and vector nematodes. It has not been studied enough about this virus in our country. There is not enough detailed research on this virus in our country. In this study, the maintenance of GFLV in vineyards and their occurrence areas were determined in Adana and Mersin provinces by serological and biological assays. Out of total 384 grapevine samples, 63 plants (16.4%) were found to be infected with GFLV by double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA). In addition to this, as herbaceous indicator plants; Nicotiana benthamiana Domin., N. tabacum L., N. rustia L., Chenopodium amaranthicolor Coste et Reyn., C. quinoa Wild., Cucumis sativus L., and Gomprena globosa L. were used in biological assays. But chloroticlocallesions were observed only on Nicotiana benthamiana Domin. among weedy indicator plants by mechanical transmission.

Keywords: GFLV, grapevine, inoculation, Nicotiana.
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