Kitchen Waste Residues as Potential Renewable Biomass Resources for the Production of Multiple Fungal Carbohydrases and Second Generation Bioethanol
Pages 186-200
Chetna Janveja, Susheel Singh Rana and Sanjeev Kumar Soni

DOI: http://dx.doi.org/10.6000/1929-6002.2013.02.02.11

Published: 20 May 2013

Abstract: Utilization of kitchen waste, the major portion of municipal solid waste for the coproduction of multiple carbohydrases and bioethanol was investigated in this study. Solid-state fermentation was performed to evaluate the potential of various steam pretreated kitchen waste residues as substrates for the coproduction of cellulolytic, hemicellulolytic, pectinolytic, amylolytic enzymes by a locally isolated strain of Aspergillus niger CJ-5. All the kitchen waste residues simply moistened with water,without the supplementationofexogenous nutrients proved good for the induction of all the enzyme components of a cocktail after96 h incubation. Of all the substrates evaluated, steam pretreated potato peels induced maximum yields corresponding to 69.0±1.92U CMCase, 16.5±0.54U FPase, 44.0±1.28U β-glucosidase, 999.0±28.90U xylanase, 58.2±2.12U mannanase, 120.0±3.72U pectinase, 31520.0±375.78U α-amylase, 482.8±9.82U glucoamylase/g dry substrate (gds). Saccharification of residues using inhouse produced crude enzyme cocktail resulted in the release of 610±10.56, 570±8.89, 435±6.54, 475±4.56, 445±4.27, 385±4.49, 370±6.89, 490±10.45 mg of total reducing sugars/g of dried potato peels, orange peels, pineapple peels, mausami peels, onion peels, banana stalks, pea pods and composite mixture respectively revealing carbohydrate conversion efficiencies in the range of 97.0-99.4%. After fermentation of released hexoses, alcohol yields ranging from 80±1.069 - 262±7.86 µL/gds were obtained.