Structural Changes to Immune Organs in Rats after Intermittent Fasting Following a High Carb and Fat Diet - Pages 6-12
Methods: An experimental study with post-test only control group design, with 15 male rat subjects aged 2.5 months were divided into three groups: first group had a diet that was ad libitum (AL); second group were given a diet high in fats and carbohydrates for 1 month then were fed ad libitum (HCL); and third group were given a diet high in fat and carbohydrates for 1 month continued with alternating 12 hour fasting periods for 72 days (F).
Results: Total Hassall’s corpuscles of AL, HCL, and F groups were 1.33 ± 077; 2.58 ± 1.35; and 0.69 ± 0.27, respectively (p = 0.008). Fat cells were not found in the thymus. The largest white pulp in the spleen was found in group F, followed by AL, while the smallest was found in group of HCL (p = 0.01). The most depleted white pulp density was the HCL group. There is no significant difference in the number of leukocytes and different leukocyte count between the groups (p> 0.05).
Conclusion: Intermittent fasting for 72 days affects the number of Hassall’s corpuscles in the thymus and the width of white pulp in the spleen of Wistar rats.
Keywords: Intermittent fasting, thymus, spleen, leukocytes, high carbohydrate diet, high fat diet.