Use of Information Parameters as Criterion for Determination of Biological Activity of Hepatoprotective Preparations
Pages 61-71
David Areshidze, Lyudmila timchenko, Maria Kozlova, Igor Rzhepakovskyand Ivan Syomin
DOI: http://dx.doi.org/10.6000/1927-5951.2016.06.02.4
Published: 27 April 2016

Abstract: Possibility of use of the information parameters characterizing adaptational and regenerative opportunities of tissue system of an organ as potential criteria for an assessment of biological activity of hepatoprotective preparations is investigated in this research. Influence of enzymatic hydrolyzate of Chlorophytum comosum (L.) on a morphofunctional condition of a liver of rats at experimental toxic damage of organ and in norm was studied. The assessment of morphofunctional condition of a liver under the influence of a preparation was carried out as with use of traditional morphological, biochemical and histologic parameters, and by means of information parameters, which were earlier used for an evaluation of the adaptative and regenerative opportunities of organs of mammals. As a result of research the hepatoprotective effect of enzymatic hydrolyzate of Chlorophytum comosum (L.) is confirmed both by dynamics of change of results of traditional techniques of an assessment and by informational parameters, that allows to draw a conclusion on possibility of use of information parameters as criterion for assesment of effectiveness of biostimulation.

Keywords: Liver, hepatoprotector, biological activity, entropy.

Enrichment of Bio-Active Phthalides in Celery Seed Oil 
Pages 250-257
Halagur B. Sowbhagya and Pullabhatla Srinivas
DOI: http://dx.doi.org/10.6000/1927-5951.2013.03.04.6
Published: 31 October 2013

Abstract: Objective: To develop an efficient process to obtain a phthalide-enriched nutraceutical fraction from celery seed oil.

Methods: Three approaches viz., fractional distillation, column chromatography and solvent-solvent partition, have been used.

Results: Fractional distillation of celery seed oil (13.7g) afforded i) a limonene-rich fraction (7.6g, 97% purity), ii) a fraction containing β-selinene (2.8 g, 90 % purity) and iii) one containing phthalides (2.9 g, 90 % purity). Solvent-solvent partition of celery seed oil gave limonene (87%) and a fraction containing phthalides (49%), which on further fractionation afforded a phthalide-enriched fraction (90%). By conventional silica gel column chromatography, a product rich in phthalides (53-74%) could be obtained.

Conclusion: Fractional distillation is shown to be a viable method to process celery seed oil into a phthalide-enriched product with high nutraceutical potential.

Biological Effects of Manganese Oxide Nanoparticles after Peroral Intake
Pages 231-237
N.V. Zaitseva, M.A. Zemlyanova, V.N. Zvezdin and T.I. Akafieva
DOI: http://dx.doi.org/10.6000/1927-5951.2013.03.04.3
Published: 31 October 2013

Abstract: Nanodispersed manganese oxide is a unique substance with a high application potential in nanoelectronics and nanooptics. The scientific literature contains little information about the biological effects and toxic action of this substance after it enters a human body. The biological effects were studied in Wistar rats after intragastric administration of manganese oxide for 30 days. The effects included loss in the body mass, activation of oxidation processes (increased level of lipid hydroperoxides, MDA in the blood serum), decrease in the antioxidant activity (inhibited antioxidant activity in the blood serum), damaged hepatocyte membranes (higher serum AST and ALT levels), and protein synthesizing liver function abnormalities (low albumins, high gamma globulins in the blood serum).

IgE-Mediated Food Allergy; Complications & Implications
Pages 238-240
Lotfollah Behroo
DOI: http://dx.doi.org/10.6000/1927-5951.2013.03.04.4
Published: 31 October 2013

Abstract: IgE-mediated allergic diseases are a major socio-economic problem caused by complex interactions between genes and the environment. In general, the production of IgE and allergic inflammation result from overexpression of T-helper 2[(Th2); IgE-producing T-lymphocyte lineage] type immune responses (type I hypersensitivity reactions) cytokines.

On the average, it is estimated that 30-40% of the world population are now, being affected by one or more allergic condition(s) of which food allergies, for the most part, account for this augmentation and consequently embrace a major portion of the affections(up to 20%). Of course, it should be noted that, the vast majority of reactions due to food allergy do not require hospitalisation and, thus, the true number of distinguished/reported cases is still, likely to be significantly higher.

Virtually, any food protein can cause clinical syndrome in susceptible individual; however, only a small number of foods are responsible for more than 90 percent of allergic food reactions, and most patients are sensitive to fewer than three foods. In children, the most common foods causing reactions are egg, milk, peanuts, soy, wheat, tree nuts and fish. Adults most often react to peanuts, tree nuts, fish and shellfish.

Despite allergy to food proteins having been known for many years, there is little information on whether food antigens are increased in these conditions and how much antigen is absorbed as well as, the biological activity of the absorbed allergen. One explanation could be the limitations of sensitive analytic methods for analyzing food proteins in human serum.

On the other part, current curative cares under evaluation include strategies to block IgE or IgE synthesis and to interrupt the Th2-dependent allergic cascade.

Altogether, the thing of noteworthy is that, a determined effort using the best appropriate food allergy diagnostic technics would be certainly required in order to, produce the most clinically effective/safe patient results and, develop any effectual/promising therapeutic approaches for IgE-mediated food allergies owing to their epidemic worldwide increase in prevalence and morbidity.